Mouse anti-Human CD11a Antibody | anti-CD11a antibody

Anti-Hu CD11a FITC

Cat. Num. AAA1801157

• Gene Names: ITGAL; CD11A; LFA-1; LFA1A
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD11a; Anti-Hu CD11a FITC; integrin subunit alpha L; LFA-1; LFA1A; Integrin alpha-L; ITGAL; anti-CD11a antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: MEM-25
• Specificity: The antibody MEM-25 reacts with an extracellular epitope of CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
• Purity/Purification: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
• Sequence Length: 1170

• Applicable Applications for anti-CD11a antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: QC tested
• Immunogen: Leukocytes from a pacient suffering from a LGL-type leukaemia.
• Conjugation: FITC
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD11a (MEM-25) FITC.)

Related Product Information for anti-CD11a antibody

CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.

NCBI and Uniprot Product Information

• NCBI GI #: 88911345
• NCBI GeneID: 3683
• UniProt Accession #: P20701
• NCBI Official Full Name: Integrin alpha-L
• NCBI Official Synonym Full Names: integrin subunit alpha L
• NCBI Official Symbol: ITGAL
• NCBI Official Synonym Symbols: CD11A; LFA-1; LFA1A
• NCBI Protein Information: integrin alpha-L
• UniProt Protein Name: Integrin alpha-L
• UniProt Gene Name: ITGAL
• UniProt Synonym Gene Names: CD11A; LFA-1A
• UniProt Entry Name: ITAL_HUMAN

NCBI Description

ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGAL: Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Belongs to the integrin alpha chain family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Cell surface

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; membrane; plasma membrane; immunological synapse; intercellular junction; integrin complex; external side of plasma membrane

Molecular Function: protein binding; protein heterodimerization activity; metal ion binding; protein complex binding; ICAM-3 receptor activity; cell adhesion molecule binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; regulation of immune response; cell-matrix adhesion; activated T cell proliferation; positive regulation of calcium-mediated signaling; signal transduction; receptor clustering; heterophilic cell adhesion; leukocyte adhesion; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; positive regulation of T cell proliferation; inflammatory response; blood coagulation; cell adhesion; cell motility; leukocyte migration