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Western Blot (WB) (Western Blot (WB) analysis of ZP1 (G259) pAb at 1:1000 dilutionLane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (20ug))

Rabbit anti-Human ZP1 Polyclonal Antibody | anti-ZP1 antibody

ZP1 (G259) Polyclonal Antibody

Gene Names
ZP1; OOMD; OOMD1; HEL163
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
ZP1; Polyclonal Antibody; ZP1 (G259) Polyclonal Antibody; Zona pellucida sperm-binding protein 1; Zona pellucida glycoprotein 1; Zp-1; anti-ZP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ZP1 (G259) polyclonal antibody detects endogenous levels of ZP1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
638
Applicable Applications for anti-ZP1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 240-280 of Human ZP1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ZP1 (G259) pAb at 1:1000 dilutionLane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (20ug))

Western Blot (WB) (Western Blot (WB) analysis of ZP1 (G259) pAb at 1:1000 dilutionLane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (20ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ZP1 (G259) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Immunohistochemistry (IHC) (Immunohistochemistry (IHC) analyzes of ZP1 (G259) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Related Product Information for anti-ZP1 antibody
The mammalian zona pellucida is composed of three major glycoproteins, ZP1, ZP2 and ZP3. ZP2 has been implicated as a secondary sperm receptor that binds sperm only after the induction of the sperm acrosome reaction. Both ZP2 and ZP3 are modified by the zona reaction; ZP2 undergoes a proteolytic cleavage and ZP3 loses its ability to induce the acrosome reaction and its sperm receptor activity. During the process of fertilization, the initial interaction between male and female gametes is mediated by a sperm receptor, ZP3, which resides in the extracellular glycoprotein matrix (zona pellucida) surrounding the oocyte

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 75kDa
NCBI Official Full Name
zona pellucida sperm-binding protein 1
NCBI Official Synonym Full Names
zona pellucida glycoprotein 1
NCBI Official Symbol
ZP1
NCBI Official Synonym Symbols
OOMD; OOMD1; HEL163
NCBI Protein Information
zona pellucida sperm-binding protein 1
UniProt Protein Name
Zona pellucida sperm-binding protein 1
UniProt Gene Name
ZP1
UniProt Synonym Gene Names
Zp-1
UniProt Entry Name
ZP1_HUMAN

NCBI Description

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene ensures the structural integrity of the zona pellucida. Mutations in this gene are a cause of oocyte maturation defect and infertility. [provided by RefSeq, May 2014]

Uniprot Description

ZP1: The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida. Belongs to the ZP domain family. ZPB subfamily.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; integral to membrane; extracellular region; plasma membrane; secretory granule

Biological Process: binding of sperm to zona pellucida; single fertilization; multicellular organism reproduction

Disease: Oocyte Maturation Defect

Research Articles on ZP1

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Product Notes

The ZP1 zp1 (Catalog #AAA3004483) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZP1 (G259) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the ZP1 zp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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