JAK3 recombinant protein

Recombinant JAK3 (781-1124) protein

Cat. Num. AAA389417

• Gene Names: JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
• Synonyms: JAK3; Recombinant JAK3 (781-1124) protein; Janus Kinase 1; JAK3 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant JAK3 (781-1124) protein is supplied in 25mM HEPESNaOH pH7.5, 500mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Protein Details: Recombinant JAK3 (781-1124) protein that includes amino acids 781-1124 of human JAK3 protein (accession number NP_000206.2) was expressed in a baculovirus expression system, and contains an N-terminal DYKDDDDK tag.
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant JAK3 (781-1124) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 39.8 kDa Purity: >80%.)

Testing Data

(HTRF assay for JAK3 (781-1124) activity 1 uM TK substrate was incubated with different concentrations of JAK3 (781-1124) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Testing Data

(HTRF assay for JAK3 (781-1124) activity 1 uM TK substrate was incubated with different concentrations of JAK3 (781-1124) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Related Product Information for JAK3 recombinant protein

Background: JAK3 (Janus Kinase 3) a non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation and mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, JAK3 plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, JAK3 phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, JAK3 phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion. Diseases associated with JAK3 include Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-Negative, B Cell-Positive, Nk Cell-Negative and Nk-Cell Enteropathy

Product Categories/Family for JAK3 recombinant protein

Recombinant Proteins; Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 47157315
• NCBI GeneID: 3718
• NCBI Accession #: NP_000206.2
• NCBI GenBank Nucleotide #: NM_000215.3
• UniProt Accession #: P52333; Q13259; Q13260; Q13611; Q99699; Q9Y6S2
• Molecular Weight: 125,099 Da
• NCBI Official Full Name: tyrosine-protein kinase JAK3
• NCBI Official Synonym Full Names: Janus kinase 3
• NCBI Official Symbol: JAK3
• NCBI Official Synonym Symbols: JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
• NCBI Protein Information: tyrosine-protein kinase JAK3; leukocyte Janus kinase; Janus kinase 3 (a protein tyrosine kinase, leukocyte)
• UniProt Protein Name: Tyrosine-protein kinase JAK3
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: JAK3
• UniProt Synonym Gene Names: JAK-3; L-JAK
• UniProt Entry Name: JAK3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008]

Uniprot Description

JAK3: a non-receptor tyrosine-kinase of the Jak family expressed predominantly in immune cells. Mediates signal transduction via the common gamma-chain of cytokine receptors, including IL-2, -4, -7, -9, and -15. Interacts with members of the STAT (signal transduction and activators of transcription) family. Interacts with STAM2 and SHB. Contains two protein kinase domains. Mutations that abrogate JAK3 function cause an autosomal SCID (severe combined immunodeficiency disease). Inhibitor: R017s for organ transplants. Three differentially spliced isoforms have been described.

Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; EC 2.7.10.2; TK group; JakA family

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; endomembrane system; cytosol

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein phosphatase binding; ATP binding; hormone receptor binding

Biological Process: negative regulation of interleukin-12 production; cell migration; peptidyl-tyrosine phosphorylation; negative regulation of T-helper 1 cell differentiation; negative regulation of interleukin-10 production; negative regulation of FasL biosynthetic process; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; regulation of apoptosis; negative regulation of dendritic cell cytokine production; tyrosine phosphorylation of STAT protein; B cell differentiation; innate immune response; erythrocyte differentiation; positive regulation of T cell proliferation; T cell homeostasis; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; negative regulation of T cell activation; transmembrane receptor protein tyrosine kinase signaling pathway; STAT protein nuclear translocation

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-negative