Guinea pig anti-Human, Mouse ZO-2 Polyclonal Antibody | anti-Tjp2 antibody

ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)

Cat. Num. AAA621922

• Gene Names: Tjp2; ZO-2; MGC124724
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Purified; Purified
• Synonyms: ZO-2; Polyclonal Antibody; ZO-2 (Tight Junction-associated Polypeptide; Zonula Occludens 2); Anti -ZO-2 (Tight Junction-associated Polypeptide; anti-Tjp2 antibody

• Host: Guinea pig
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Recognizes human zona occludens 2 (ZO-2), an ~160kD tight junction protein belonging
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

• Applicable Applications for anti-Tjp2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry.; Dilution: Immunofluorescence: 1:50; Western Blot: 1:50; Immunohistochemistry (frozen): 1:50
• Immunogen: ZO-2, aa 411-467.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Tjp2 antibody

Human zona occludens 2 (ZO-2), an ~160kD tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-2, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes.

ZO-2 can interact with zona occludens 1 (ZO-1). Furthermore, the PDZ2 domain of ZO-2 was shown to interact with connexin-43, the predominant connexin in epithelial and most other tissues which is involved in cell growth control and embryonic development.

Product Categories/Family for anti-Tjp2 antibody

Antibodies; Abs to Connexin; Junction Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 1839162
• NCBI GeneID: 115769
• UniProt Accession #: P70625; P97625; Q3ZB99
• Molecular Weight: 90,777 Da
• NCBI Official Full Name: zonula occludens 2 protein
• NCBI Official Synonym Full Names: tight junction protein 2
• NCBI Official Symbol: Tjp2
• NCBI Official Synonym Symbols: ZO-2; MGC124724
• NCBI Protein Information: tight junction protein ZO-2
• UniProt Protein Name: Zonula occludens 2 protein
• Protein Family: Tight junction protein
• UniProt Gene Name: Tjp2
• UniProt Synonym Gene Names: ZO-2
• UniProt Entry Name: P70625_RAT

NCBI Description

a tight junction protein that may be involved in carotid neointimal formation [RGD]

Uniprot Description

ZO2: a scaffolding protein of the MAGUK (membrane-associated guanylate kinase) family associated with epithelial tight junctions (TJ). May mediate dynamic changes in the composition of TJs. Plays a role in tight junctions and adherens junctions. Forms homodimers, and heterodimer with ZO-1 and ZO-3. The ZOs shuttle between the TJ and the nucleus, where they may regulate gene expression. Interacts with occluding, UBN1 and scribble. Interacts with SAFB in the nucleus. TJ, or zonula occludens, are the closely associated areas of two cells whose membranes are tightly joined together. They are composed of a branching network of strands, the efficiency of which increases exponentially with the number of strands. The strands associate with transmembrane proteins that bind to similar proteins on adjacent cells, and with submembranous proteins that are anchored to the actin component of the cytoskeleton. Thus, TJs join together the cytoskeletons of adjacent cells. They endow tissues with substantial form, shape and location, enforce cellular polarity, and form barriers to molecules and pathogens. Molecules forming the membranous part of TJs include occludin, claudins, tricellulin and junctional adhesion molecules. These molecules interact with the proteins ZO-1, ZO-2 and ZO-3. Tight junction proteins are involved in the epithelial-mesenchymal transitions (EMT) in tumors. Five isoforms of the human protein are produced by alternative promoters. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues..

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Cellular Component: tight junction; cytoplasm; plasma membrane; cell junction

Molecular Function: protein binding, bridging; protein C-terminus binding; protein domain specific binding

Biological Process: response to organic substance; intestinal absorption