Rabbit anti-Dog, Mouse ZO-1 Polyclonal Antibody | anti-ZO-1 antibody

ZO-1, Human, pAb

Cat. Num. AAA584518

• Gene Names: Tjp1; ZO1
• Reactivity: Dog, Mouse
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: ZO-1; Polyclonal Antibody; Human; pAb; Tight junction protein 1 (TJP1); anti-ZO-1 antibody

• Host: Rabbit
• Reactivity: Dog, Mouse
• Clonality: Polyclonal
• Isotype: Ig
• Purity/Purification: Protein A
• Form/Format: 0.2 um filtered in PBS+0.1%BSA+0.02%NaN3; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Concentration: 100 ug/ml (varies by lot)

• Applicable Applications for anti-ZO-1 antibody: Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. ; The typical starting working dilution is 1:50.; • IF: For Immunofluorescence intracellular staining is required. ; Cells were fixed with 4% paraformaldehyde and cells were permeabilized with PBS, 0.1% saponin and 0.5% BSA.; • FC: For intracellular staining Huvec cells were permeabilized with buffer containing 0.1% saponin and 0.5% BSA.
• Conjugate: None
• Immunogen: Recombinant human GST-ZO-1 (aa 1-888)
• Preparation and Storage: Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.

Immunofluorescence (IF)

(Immunofluorescence staining in Huvec cells.)

Flow Cytometry (FC/FACS)

(Flow Cytometry with Huvec cells. The black line represents the cells only, the red line the control and the blue line MBS584518 in a concentration of 1 ug/250000 cells.)

Related Product Information for anti-ZO-1 antibody

The polyclonal antibody recognizes human zona occludens 1 (ZO-1), an ~220 kDa tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions. The polyclonal antibody was raised against amino acids 432-1150 of human ZO-1 and recognizes both ZO-1 alpha-minus and alpha-plus. It cross reacts with mouse and dog ZO-1.

NCBI and Uniprot Product Information

• NCBI GI #: 303710
• NCBI GeneID: 21872
• NCBI Official Full Name: ZO-1
• NCBI Official Synonym Full Names: tight junction protein 1
• NCBI Official Symbol: Tjp1
• NCBI Official Synonym Symbols: ZO1
• NCBI Protein Information: tight junction protein ZO-1; zona occludens protein 1; zonula occludens protein 1
• UniProt Protein Name: Tight junction protein ZO-1
• UniProt Gene Name: Tjp1
• UniProt Synonym Gene Names: Zo1
• UniProt Entry Name: ZO1_MOUSE

Uniprot Description

ZO1: a scaffolding protein of the MAGUK (membrane-associated guanylate kinase) family associated with tight junctions (TJ). May mediate dynamic changes in the composition of TJs. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting the protein kinase MRCKb to the leading edge of migrating cells. Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with HSPA4 and KIRREL1. Forms homodimers and heterodimers with ZO-2 or ZO-3. Interacts with occludin, CALM, claudins, CGN, CXADR, GJA12, GJD3 and UBN1. Interacts (via ZU5 domain) with MRCKb and MYZAP. Interacts (via PDZ domain) with Cx43. ZO-2 and ZO-3 shuttle between the TJ and the nucleus, where they may regulate gene expression. TJ, or zonula occludens, are the closely associated areas of two cells whose membranes are tightly joined together. They are composed of a branching network of strands, the efficiency of which increases exponentially with the number of strands. The strands associate with transmembrane proteins that bind to similar proteins on adjacent cells, and with submembranous proteins that are anchored to the actin component of the cytoskeleton. Thus, TJs join together the cytoskeletons of adjacent cells. They endow tissues with substantial form, shape and location, enforce cellular polarity, and form barriers to molecules and pathogens. Molecules forming the membranous part of TJs include occludin, claudins, tricellulin and junctional adhesion molecules. These molecules interact with the proteins ZO-1, ZO-2 and ZO-3. Tight junction proteins can be up- or downregulated in cancer and are involved in the epithelial-mesenchymal transitions (EMT) in tumors. Alternative splicing produces two isoforms. The long isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Cellular Component: apical junction complex; intercellular canaliculus; cell surface; tight junction; apicolateral plasma membrane; basolateral plasma membrane; intercellular junction; adherens junction; cell-cell adherens junction; membrane; apical part of cell; apical plasma membrane; cytoplasm; plasma membrane; gap junction; nucleus; cell junction

Molecular Function: calmodulin binding; protein C-terminus binding; protein domain specific binding; protein binding

Biological Process: sensory perception of sound; blastocyst formation