Rabbit ZO-1 Polyclonal Antibody | anti-ZO-1 antibody

ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1) (MaxLight 405)

Cat. Num. AAA6506603

• Reactivity: Human, Canine, Guinea Pig, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ZO-1; Polyclonal Antibody; ZO-1 (Tight Junction-associated Polypeptide; Zonula Occludens 1) (MaxLight 405); anti-ZO-1 antibody

• Host: Rabbit
• Reactivity: Human, Canine, Guinea Pig, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ZO-1 alpha+ and ZO-1 alpha-isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western Blots, but potential reactivity with these proteins has not been fully characterized. Species Crossreactivtiy: mouse, rat, guinea pig, canine. Moderate reactivity with chicken.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight405.

• Applicable Applications for anti-ZO-1 antibody: FLISA, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: A 69kD fusion protein corresponding to aa463-1109 of human ZO-1 cDNA. This sequence lies from the N-terminal to the 80aa region (the alpha-motif) present in the a+isoform but absent in the alpha-isoform due to alternative splicing.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDCK cells usingMBS618099.)

Western Blot (WB)

(Western Blot analysis of Caco-2 cell lysates usingMBS618099.)

Immunofluorescence (IF)

(Indirect immunofluorescence staining of rat glomerulus using MBS618099 (red). Image courtesy of Hidetake Kurihara, PhD, Juntendo University School of Medicine, Tokyo, Japan)

Product Categories/Family for anti-ZO-1 antibody

Antibodies; Connexin; Junction Proteins

References

1. Willott, E. et al., Am J Physiol. 262 (Cell Physiol. 31):C1119-C1124 (1992). 2. Willott, E. et al., Proc. Natl. Acad. Sci., USA 90(16):7834-7838 (1993). 3. Balda, M.S. and Anderson, J.M., Am J Physiol 264 (Cell Physiol. 33):C918-C924 (1993). 4. Tsarfaty, I, et al., Science 263:98-101 (1994). 5. Keon, BH, et al., J. Cell Biol. 134(4):1003-1018 (1996). 6. Bomsel, M. Nature Medicine 3(1):42-47 (1997). 7. Watabe-Uchida, M, et al., J. Cell Biol 142(3):847-857 (1998). 8. Hoover, KB, et al., J. Am. J. Pathol 153(6):1767-1773 (1998). 9. Rajasekaran, AK, et al., J. Cell Biol 132(3):451-463 (1996). 10. Haskins, J, et al., J. Cell Biol. 141(1):199-208 (1998). 11. Kachinsky, AM, et al., J. Cell Biol. 145:391-402 (1999). 12. Wu, S., et al., J. Proc. Natl. Acad. Sci. 95(25):14979-14984 (1998). 13. Moreira, JE, et al. J. Histochem. Cytochem. 46(7):847-854 (1998).14. Potempa, S. and Ridley, A.J. Mol Biol Cell 9:2185-2200 (1998).15. Mann, B, et al., Proc. Natl. Acad. Sci. 96:1603-1608 (1999).16. Laing, JG, et al., J. Biol. Chem. 276 (25): 23051-23055 (2001). 17. Hirata, K., et al., J. Biol. Chem. 276 (19): 16223-16231 (2001).18. Toyofuku, T., et al., J. Biol. Chem. 276 (3): 1780-1788 (2001).19. Singh, U., et al., J. Biol. Chem. 275 (24): 18407-18417 (2000).20. Bazzoni, G., et al., J. Biol. Chem. 275 (27): 20520-20526 (2000).21. Fluge, O., et al., World J. Surg. 25: 623-631 (2001).22. Hamazaki, Y., et al., J. Biol. Chem. 277 (1): 455-461 (2002).23. Duffy, H.S., et al., J. Neuroscience 20:RC114(1-6).