Rabbit anti-Human ZNF9 Polyclonal Antibody | anti-ZNF9 antibody

ZNF9, ID (CNBP, RNF163, ZNF9, Cellular nucleic acid-binding protein, Zinc finger protein 9) (Azide free) (HRP)

Cat. Num. AAA6367970

• Gene Names: CNBP; DM2; ZNF9; CNBP1; PROMM; RNF163; ZCCHC22
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZNF9; Polyclonal Antibody; ID (CNBP; RNF163; Cellular nucleic acid-binding protein; Zinc finger protein 9) (Azide free) (HRP); CNBP; Zinc finger protein 9; anti-ZNF9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ZNF9 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ZNF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74~104 amino acids from the Central region of human ZNF9.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ZNF9 Antibody (Center) in Neuro-2a cell line lysates (35ug/lane). ZNF9 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(ZNF9 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZNF9 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ZNF9 antibody

ZNF9 encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion in the first intron of this gene results in myotonic dystrophy type 2.

Product Categories/Family for anti-ZNF9 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Lucchiari, S., et al. J. Neurol. Sci. 275 (1-2), 159-163 (2008). Auvinen, S., et al. Arthritis Rheum. 58(11):3627-3631(2008). Gerbasi, V.R., et al. Mol. Cell Proteomics 6(6):1049-1058(2007). Toth, C., et al. Muscle Nerve 35(2):259-264(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 187608726
• NCBI GeneID: 7555
• NCBI Accession #: NP_001120664.1
• NCBI GenBank Nucleotide #: NM_001127192.1
• UniProt Accession #: P62633; P20694; Q4JGY0; Q4JGY1; Q5QJR0; A8K7V4; B2RAV9; B4DP17; D3DNB9; D3DNC0; D3DNC1; E9PDR7
• Molecular Weight: 18,871 Da
• NCBI Official Full Name: cellular nucleic acid-binding protein isoform 1
• NCBI Official Synonym Full Names: CCHC-type zinc finger, nucleic acid binding protein
• NCBI Official Symbol: CNBP
• NCBI Official Synonym Symbols: DM2; ZNF9; CNBP1; PROMM; RNF163; ZCCHC22
• NCBI Protein Information: cellular nucleic acid-binding protein; cellular nucleic acid binding protein; erythroid differentiation-related; sterol regulatory element-binding protein; zinc finger protein 273; zinc finger protein 9 (a cellular retroviral nucleic acid binding protein)
• UniProt Protein Name: Cellular nucleic acid-binding protein
• UniProt Gene Name: CNBP
• UniProt Synonym Gene Names: RNF163; ZNF9; CNBP
• UniProt Entry Name: CNBP_HUMAN

NCBI Description

This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion in the first intron of this gene results in myotonic dystrophy type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ZNF9: Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression. Defects in CNBP are the cause of dystrophia myotonica type 2 (DM2); also known as proximal myotonic myopathy (PROMM). A multisystem disease characterized by the association of proximal muscle weakness with myotonia, cardiac manifestations and cataract. Additional features can include hyperhidrosis, testicular atrophy, insulin resistance and diabetes and central nervous system anomalies in rare cases. The causative mutation is a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the CNBP gene. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; RNA-binding; DNA-binding

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: endoplasmic reticulum; nucleus; cytosol

Molecular Function: protein binding; single-stranded RNA binding; zinc ion binding; transcription factor activity; single-stranded DNA binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; cholesterol biosynthetic process

Disease: Myotonic Dystrophy 2