Chemiluminescence
((0.3ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat ZNF364 Polyclonal Antibody
ZNF364 (E3 Ubiquitin-protein Ligase RNF115, RING Finger Protein 115, Rabring 7, Zinc Finger Protein 364, RNF115)
Reactivity
Canine, Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
ZNF364; Polyclonal Antibody; ZNF364 (E3 Ubiquitin-protein Ligase RNF115; RING Finger Protein 115; Rabring 7; Zinc Finger Protein 364; RNF115); Anti -ZNF364 (E3 Ubiquitin-protein Ligase RNF115; anti-ZNF364 antibody
Host
Goat
Reactivity
Canine, Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes human RNF115. Species sequence homology: Canine, mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Applicable Applications for anti-ZNF364 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:32,000
Western Blot: 0.3-1ug/ml, observed in human lysates of cell line HEPG2 on ~35kD bands
Dilution: ELISA: 1:32,000
Western Blot: 0.3-1ug/ml, observed in human lysates of cell line HEPG2 on ~35kD bands
Immunogen
Synthetic peptide corresponding to C-DQDNRANERGHQTH, from human RNF115, at the internal region of the protein (NP_055270.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chemiluminescence
((0.3ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Chemiluminescence
((0.3ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Related Product Information for anti-ZNF364 antibody
Acts as an E2-dependent E3 ubiquitin-protein ligase. May be involved in endocytic trafficking.
Product Categories/Family for anti-ZNF364 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
zinc finger protein 364
Uniprot Description
ZNF364: Acts as an E2-dependent E3 ubiquitin-protein ligase. May be involved in endocytic trafficking.
Protein type: EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system; Ligase; EC 6.3.2.-
Chromosomal Location of Human Ortholog: 1q21.1
Cellular Component: cytosol
Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; protein autoubiquitination; ubiquitin-dependent protein catabolic process via the multivesicular body pathway
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Product Notes
The ZNF364 (Catalog #AAA6007546) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The ZNF364 (E3 Ubiquitin-protein Ligase RNF115, RING Finger Protein 115, Rabring 7, Zinc Finger Protein 364, RNF115) reacts with Canine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF364 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:32,000 Western Blot: 0.3-1ug/ml, observed in human lysates of cell line HEPG2 on ~35kD bands. Researchers should empirically determine the suitability of the ZNF364 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZNF364, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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