Western Blot (WB)
(ZNF313 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ZNF313 antibody detected the ZNF313 protein (arrow).)
Rabbit anti-Human ZNF313 Polyclonal Antibody | anti-ZNF313 antibody
ZNF313, CT (RNF114, ZNF228, ZNF313, RING finger protein 114, Zinc finger protein 228, Zinc finger protein 313) (PE)
Gene Names
RNF114; ZNF313; PSORS12
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ZNF313; Polyclonal Antibody; CT (RNF114; ZNF228; RING finger protein 114; Zinc finger protein 228; Zinc finger protein 313) (PE); RNF114; Zinc finger protein 313; anti-ZNF313 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-ZNF313 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ZNF313 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-203 amino acids from the C-terminal region of human ZNF313.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(ZNF313 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ZNF313 antibody detected the ZNF313 protein (arrow).)
Western Blot (WB)
(ZNF313 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ZNF313 antibody detected the ZNF313 protein (arrow).)
Immunohistochemistry (IHC)
(ZNF313 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZNF313 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ZNF313 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZNF313 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-ZNF313 antibody
ZNF313 is a novel human zinc finger protein gene that contains both ring finger and C(2)H(2) domain. It may play a role in spermatogenesis.
Product Categories/Family for anti-ZNF313 antibody
References
Capon, F., et al. Hum. Mol. Genet. 17(13):1938-1945(2008). Ma, Y.X., et al. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 35(3):230-237(2003). Deloukas, P., et al. Nature 414(6866):865-871(2001). Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,004 Da
NCBI Official Full Name
Homo sapiens ring finger protein 114 (RNF114), mRNA
NCBI Official Synonym Full Names
ring finger protein 114
NCBI Official Symbol
RNF114
NCBI Official Synonym Symbols
ZNF313; PSORS12
NCBI Protein Information
E3 ubiquitin-protein ligase RNF114; zinc finger protein 228; zinc finger protein 313
UniProt Protein Name
E3 ubiquitin-protein ligase RNF114
UniProt Gene Name
RNF114
UniProt Synonym Gene Names
ZNF228; ZNF313
UniProt Entry Name
RN114_HUMAN
Uniprot Description
ZNF313: May play a role in spermatogenesis. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system; Transcription factor; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 20q13.13
Cellular Component: cytoplasm; intracellular; nucleus
Molecular Function: zinc ion binding; ligase activity
Biological Process: multicellular organismal development; protein ubiquitination; spermatogenesis; cell differentiation
Research Articles on ZNF313
Similar Products
Product Notes
The ZNF313 rnf114 (Catalog #AAA6366161) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZNF313, CT (RNF114, ZNF228, ZNF313, RING finger protein 114, Zinc finger protein 228, Zinc finger protein 313) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF313 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-100 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ZNF313 rnf114 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZNF313, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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