Human Artemin (ARTN) ELISA Kit | ARTN elisa kit

Human Artemin (ARTN) ELISA Kit

Cat. Num. AAA2019300

• Gene Names: ARTN; EVN; NBN; ENOVIN
• Reactivity: Human
• Synonyms: Artemin (ARTN); Human Artemin (ARTN) ELISA Kit; NBN; EVN; ENOVIN; Neublastin; Neurotrophic Factor; ARTN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Artemin (ARTN).; No significant cross-reactivity or interference between Artemin (ARTN) and analogues was observed.
• Sequence Length: 237

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Artemin (ARTN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Artemin (ARTN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ARTN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Artemin (ARTN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Artemin (ARTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Artemin (ARTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Artemin (ARTN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ARTN elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4235100
• NCBI GeneID: 9048
• UniProt Accession #: Q5T4W7; O95441; O96030; Q6P6A3; D3DPY1; D3DPY3
• Molecular Weight: 23,616 Da
• NCBI Official Full Name: Artemin
• NCBI Official Synonym Full Names: artemin
• NCBI Official Symbol: ARTN
• NCBI Official Synonym Symbols: EVN; NBN; ENOVIN
• NCBI Protein Information: artemin
• UniProt Protein Name: Artemin
• UniProt Gene Name: ARTN
• UniProt Synonym Gene Names: EVN
• UniProt Entry Name: ARTN_HUMAN

NCBI Description

The protein encoded by this gene is a member of the glial cell line-derived neurotophic factor (GDNF) family of ligands which are a group of ligands within the TGF-beta superfamily of signaling molecules. GDNFs are unique in having neurotrophic properties and have potential use for gene therapy in neurodegenerative disease. Artemin has been shown in culture to support the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons. This protein is a ligand for the RET receptor and uses GFR-alpha 3 as a coreceptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

artemin: Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Belongs to the TGF-beta family. GDNF subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: extracellular space

Molecular Function: growth factor activity; receptor binding

Biological Process: axon guidance; neuroblast proliferation; signal transduction; induction of positive chemotaxis; peripheral nervous system development