Rabbit anti-Human ZNF197 Polyclonal Antibody | anti-ZNF197 antibody

ZNF197, NT (ZNF197, ZKSCAN9, ZNF166, Zinc finger protein 197, Zinc finger protein with KRAB and SCAN domains 9, ZnF20, pVHL-associated KRAB domain-containing protein) (Azide free) (HRP)

Cat. Num. AAA6365803

• Gene Names: ZNF197; P18; VHLaK; ZNF20; ZNF166; ZKSCAN9; ZSCAN41; D3S1363E
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZNF197; Polyclonal Antibody; NT (ZNF197; ZKSCAN9; ZNF166; Zinc finger protein 197; Zinc finger protein with KRAB and SCAN domains 9; ZnF20; pVHL-associated KRAB domain-containing protein) (Azide free) (HRP); pVHL-associated KRAB domain-containing protein; anti-ZNF197 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ZNF197 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ZNF197 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 321-351 amino acids from the N-terminal region of human ZNF197.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ZNF197 Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ZNF197 antibody detected the ZNF197 protein (arrow).)

Immunohistochemistry (IHC)

(ZNF197 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZNF197 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ZNF197 antibody

ZNF197 product belongs to the zinc finger protein superfamily, members of which are regulatory proteins characterized by nucleic acid-binding zinc finger domains. The encoded protein contains 20 tandemly arrayed C2H2-type zinc fingers, a Kruppel-associated box (KRAB) domain, and a SCAN box. This transcript turns over rapidly and contains 3' UTR AUUUA motifs, which are often a hallmark of rapid turnover. It is overexpressed in some thyroid papillary carcinomas. ZNF197 is located in a cluster of zinc finger genes at 3p21. Two alternatively spliced transcripts encoding different isoforms have been described.

Product Categories/Family for anti-ZNF197 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Li, Z., et al. EMBO J. 22(8):1857-1867(2003). Gonsky, R., et al. Nucleic Acids Res. 25(19):3823-3831(1997). Calabro, V., et al. Hum. Genet. 95(1):18-21(1995) Pengue, G., et al. Hum. Mol. Genet. 2(6):791-796(1993).

NCBI and Uniprot Product Information

• NCBI GI #: 11386193
• NCBI GeneID: 10168
• NCBI Accession #: NP_008922.1
• NCBI GenBank Nucleotide #: NM_006991.4
• UniProt Accession #: O14709; Q86VG0; B2RAH8
• Molecular Weight: 30,229 Da
• NCBI Official Full Name: zinc finger protein 197 isoform 1
• NCBI Official Synonym Full Names: zinc finger protein 197
• NCBI Official Symbol: ZNF197
• NCBI Official Synonym Symbols: P18; VHLaK; ZNF20; ZNF166; ZKSCAN9; ZSCAN41; D3S1363E
• NCBI Protein Information: zinc finger protein 197
• UniProt Protein Name: Zinc finger protein 197
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZNF197
• UniProt Synonym Gene Names: ZKSCAN9; ZNF166
• UniProt Entry Name: ZN197_HUMAN

NCBI Description

This gene product belongs to the zinc finger protein superfamily, members of which are regulatory proteins characterized by nucleic acid-binding zinc finger domains. The encoded protein contains 20 tandemly arrayed C2H2-type zinc fingers, a Kruppel-associated box (KRAB) domain, and a SCAN box. This transcript turns over rapidly and contains 3' UTR AUUUA motifs, which are often a hallmark of rapid turnover. It is overexpressed in some thyroid papillary carcinomas. This gene is located in a cluster of zinc finger genes at 3p21. [provided by RefSeq, Apr 2016]

Uniprot Description

ZNF197: May be involved in transcriptional regulation. Belongs to the krueppel C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent