Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.heart section using ZNF175 Antibody (N-term). MBS9201789 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Rabbit anti-Human ZNF175 Polyclonal Antibody | anti-ZNF175 antibody
ZNF175 Antibody (N-term)
Gene Names
ZNF175; OTK18
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ZNF175; Polyclonal Antibody; ZNF175 Antibody (N-term); Zinc finger protein 175; Zinc finger protein OTK18; anti-ZNF175 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ZNF175 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 150-187 amino acids from the N-terminal region of human ZNF175.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
711
Applicable Applications for anti-ZNF175 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
IHC~~1:25
WB~~1:1000
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.heart section using ZNF175 Antibody (N-term). MBS9201789 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.heart section using ZNF175 Antibody (N-term). MBS9201789 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Western Blot (WB)
(Western blot analysis of lysates from A431, Jurkat, K562, MDA-MB-231, U-251 MG cell line (from left to right), using ZNF175 Antibody (N-term). MBS9201789 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from A431, Jurkat, K562, MDA-MB-231, U-251 MG cell line (from left to right), using ZNF175 Antibody (N-term). MBS9201789 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Related Product Information for anti-ZNF175 antibody
Down-regulates the expression of several chemokine receptors. Interferes with HIV-1 replication by suppressing Tat- induced viral LTR promoter activity.
Product Categories/Family for anti-ZNF175 antibody
References
Saito H.,et al.Genomics 31:376-379(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Carlson K.A.,et al.J. Immunol. 172:381-391(2004).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Carlson K.A.,et al.J. Immunol. 172:381-391(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
81609
NCBI Official Full Name
zinc finger protein 175
NCBI Official Synonym Full Names
zinc finger protein 175
NCBI Official Symbol
ZNF175
NCBI Official Synonym Symbols
OTK18
NCBI Protein Information
zinc finger protein 175
UniProt Protein Name
Zinc finger protein 175
Protein Family
UniProt Gene Name
ZNF175
UniProt Entry Name
ZN175_HUMAN
Uniprot Description
ZNF175: Down-regulates the expression of several chemokine receptors. Interferes with HIV-1 replication by suppressing Tat- induced viral LTR promoter activity. Belongs to the krueppel C2H2-type zinc-finger protein family.
Protein type: C2H2-type zinc finger protein; DNA-binding
Chromosomal Location of Human Ortholog: 19q13.4
Cellular Component: nucleoplasm; intermediate filament cytoskeleton; cytoplasm
Molecular Function: DNA binding; metal ion binding; transcription factor activity
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; defense response to virus
Research Articles on ZNF175
Similar Products
Product Notes
The ZNF175 znf175 (Catalog #AAA9201789) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZNF175 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF175 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). IHC~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the ZNF175 znf175 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZNF175, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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