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Western Blot (WB) (INDO Antibody (Center) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the INDO antibody detected the INDO protein (arrow).)

Rabbit anti-Human, Mouse INDO Polyclonal Antibody | anti-INDO antibody

INDO, ID (IDO1, IDO, INDO, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase) (MaxLight 550)

Gene Names
IDO1; IDO; INDO; IDO-1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
INDO; Polyclonal Antibody; ID (IDO1; IDO; Indoleamine 2; 3-dioxygenase 1; Indoleamine-pyrrole 2; 3-dioxygenase) (MaxLight 550); IDO1; 3-dioxygenase; anti-INDO antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-INDO antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
INDO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the Central region of human INDO.
Conjugate
MaxLight550
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(INDO Antibody (Center) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the INDO antibody detected the INDO protein (arrow).)

Western Blot (WB) (INDO Antibody (Center) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the INDO antibody detected the INDO protein (arrow).)

Western Blot (WB)

(INDO Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the INDO antibody detected the INDO protein (arrow).)

Western Blot (WB) (INDO Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the INDO antibody detected the INDO protein (arrow).)

Immunohistochemistry (IHC)

(INDO antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the INDO antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (INDO antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the INDO antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-INDO antibody
MaxLight 550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor546, 555, DyLight549, Cy3, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Product Categories/Family for anti-INDO antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45,326 Da
NCBI Official Full Name
indoleamine 2,3-dioxygenase 1
NCBI Official Synonym Full Names
indoleamine 2,3-dioxygenase 1
NCBI Official Symbol
IDO1
NCBI Official Synonym Symbols
IDO; INDO; IDO-1
NCBI Protein Information
indoleamine 2,3-dioxygenase 1
UniProt Protein Name
Indoleamine 2,3-dioxygenase 1
Protein Family
UniProt Gene Name
IDO1
UniProt Synonym Gene Names
IDO; INDO; IDO-1
UniProt Entry Name
I23O1_HUMAN

NCBI Description

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]

Uniprot Description

INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family.

Protein type: Amino Acid Metabolism - tryptophan; Cell cycle regulation; EC 1.13.11.52; Oxidoreductase

Chromosomal Location of Human Ortholog: 8p12-p11

Cellular Component: stereocilium bundle; smooth muscle contractile fiber; cytosol

Molecular Function: electron carrier activity; metal ion binding; heme binding; indoleamine 2,3-dioxygenase activity; tryptophan 2,3-dioxygenase activity

Biological Process: regulation of activated T cell proliferation; positive regulation of T-helper 2 type immune response; negative regulation of interleukin-10 production; positive regulation of chronic inflammatory response; tryptophan catabolic process to kynurenine; positive regulation of interleukin-12 production; response to lipopolysaccharide; female pregnancy; negative regulation of T cell proliferation; cytokine production during acute inflammatory response; multicellular organismal response to stress; tryptophan catabolic process; positive regulation of T cell tolerance induction

Research Articles on INDO

Similar Products

Product Notes

The INDO ido1 (Catalog #AAA6310494) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The INDO, ID (IDO1, IDO, INDO, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase) (MaxLight 550) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's INDO can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-100 FLISA: 1:1,000 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the INDO ido1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "INDO, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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