Rabbit ZNF174 Polyclonal Antibody | anti-ZNF174 antibody

ZNF174 (E209) Polyclonal Antibody

Cat. Num. AAA3002151

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ZNF174; Polyclonal Antibody; ZNF174 (E209) Polyclonal Antibody; AW-1; AW1; Zinc finger and SCAN domain containing protein 8; Zinc finger and SCAN domain-containing protein 8; Zinc finger protein 174; ZSCAN8; anti-ZNF174 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ZNF174 (E209) polyclonal antibody detects endogenous levels of ZNF174 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 234

• Applicable Applications for anti-ZNF174 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 168-228 of Human ZNF174.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ZNF174 (E209) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.)

Related Product Information for anti-ZNF174 antibody

ZNF174, also known as ZSCAN8 or AW-1, is a transcriptional repressor that belongs to the Krueppel C2H2-type zinc-finger protein family. It is expressed in a number of different tissues, including small intestine, prostate, colon, spleen, pancreas, skeletal muscle, brain, heart, kidney and thymus, but it is most predominantly found in adult ovary and testis. ZNF174 specifically acts to repress the promoter activities of PDGF-B and TGFbeta1. ZNF174 localizes to the nucleus and contains three C2H2-type zinc fingers at the C-terminus and one SCAN domain near the N-terminus. SCAN domains are found in a number of zinc-finger proteins and are characterized by a conserved region of 84 residues. The SCAN domain seemingly regulates the association of proteins containing SCAN domains into noncovalent complexes and may also function as an underlying mechanism in selective oligomerization of these proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 73808088
• NCBI Accession #: NP_001027463.1
• NCBI GenBank Nucleotide #: NP_001027463.1
• UniProt Accession #: Q15697
• Molecular Weight: ~ 46kDa
• NCBI Official Full Name: zinc finger protein 174 isoform b
• UniProt Protein Name: Zinc finger protein 174
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZNF174
• UniProt Synonym Gene Names: ZSCAN8
• UniProt Entry Name: ZN174_HUMAN

Uniprot Description

ZNF174: a transcriptional repressor of the krueppel C2H2-type zinc-finger protein family. Two splice variant isoforms have been described.

Protein type: Transcription factor; C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; actin cytoskeleton

Molecular Function: protein homodimerization activity; metal ion binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent