Rabbit anti-Mouse Dapk3 Polyclonal Antibody | anti-Dapk3 antibody

Dapk3, ID (Dapk3, Zipk, Death-associated protein kinase 3, DAP-like kinase, MYPT1 kinase, ZIP-kinase) (MaxLight 550)

Cat. Num. AAA6290811

• Gene Names: Dapk3; dlk; ZIPK
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Dapk3; Polyclonal Antibody; ID (Dapk3; Zipk; Death-associated protein kinase 3; DAP-like kinase; MYPT1 kinase; ZIP-kinase) (MaxLight 550); ZIP-kinase; anti-Dapk3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Dapk3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
• Sequence Length: 448

• Applicable Applications for anti-Dapk3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 255-282 in the central region of mouse Dapk3, UniProt Accession #NP_031854.1; NP_001177402.1; O54784; O88764. Species sequence homology: rat
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse testis tissue lysates (35ug/lane) usingMBS6011150. This demonstrates that MBS6011150 detected the Dapk3 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue using MBS6011150 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6011150 for immunohistochemistry.)

Related Product Information for anti-Dapk3 antibody

Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection (By similarity).

Product Categories/Family for anti-Dapk3 antibody

Antibodies; Protein Kinases

References

1. Chang, A.N., et al. J. Biol. Chem. 285(8):5122-5126(2010). 2. Ohbayashi, N., et al. Biochem. Biophys. Res. Commun. 372(4):708-712(2008). 3. Wooldridge, A.A., et al. J. Biol. Chem. 283(17):11850-11859(2008). 4. Shoval, Y., et al. PLoS Genet. 3(10):1884-1893(2007). 5. Sato, N., et al. Int. Immunol. 17(12):1543-1552(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 6681133
• NCBI GeneID: 13144
• NCBI Accession #: NP_031854.1; NP_001177402.1
• NCBI GenBank Nucleotide #: NP_031854.1
• UniProt Accession #: O54784; O88764
• NCBI Official Full Name: death-associated protein kinase 3 isoform b
• NCBI Official Synonym Full Names: death-associated protein kinase 3
• NCBI Official Symbol: Dapk3
• NCBI Official Synonym Symbols: dlk; ZIPK
• NCBI Protein Information: death-associated protein kinase 3
• UniProt Protein Name: Death-associated protein kinase 3
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: Dapk3
• UniProt Synonym Gene Names: Zipk; DAP kinase 3; Dlk

Uniprot Description

Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in formation of promyelocytic leukemia protein nuclear body (PML-NB). Involved in apoptosis involving PAWR which mediates cytoplasmic relocation; in vitro phosphorylates PAWR (). Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton such as in regulation of cell polarity and cell migration. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2; disrupts the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates STAT3 and enhances its transcriptional activity. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis (). Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition.