Western Blot (WB)
(ZNF146 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ZNF146 antibody detected the ZNF146 protein (arrow).)
Rabbit anti-Human, Mouse ZNF146 Polyclonal Antibody | anti-ZNF146 antibody
ZNF146, NT (ZNF146, OZF, Zinc finger protein OZF, Only zinc finger protein, Zinc finger protein 146) (FITC)
Gene Names
ZNF146; OZF
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ZNF146; Polyclonal Antibody; NT (ZNF146; OZF; Zinc finger protein OZF; Only zinc finger protein; Zinc finger protein 146) (FITC); Zinc finger protein 146; anti-ZNF146 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Sequence Length
292
Applicable Applications for anti-ZNF146 antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ZNF146 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human ZNF146.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(ZNF146 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ZNF146 antibody detected the ZNF146 protein (arrow).)
Western Blot (WB)
(ZNF146 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ZNF146 antibody detected the ZNF146 protein (arrow).)
Western Blot (WB)
(ZNF146 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ZNF146 antibody detected the ZNF146 protein (arrow).)
Western Blot (WB)
(ZNF146 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ZNF146 antibody detected the ZNF146 protein (arrow).)
Flow Cytometry (FC/FACS)
(ZNF146 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ZNF146 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ZNF146 antibody
The ZNF146 protein is a 33 kDa Kruppel protein, composed solely of 10 zinc finger motifs. ZNF146 overexpression in tumours may alter the balance between hRap1 and other telomeric proteins; therefore ZNF146 function may be linked to telomere regulation. ZNF146 is strongly overexpressed in many pancreas and colorectal cancers. Increased gene copy numbers are detected in 3 of 12 tumor cell lines and 2 of 12 primary pancreatic carcinomas. ZNF146 is overexpresseed in 80% of colorectal cancers.
Product Categories/Family for anti-ZNF146 antibody
References
Antoine, K., et al. J. Cell. Biochem. 95(4):763-768(2005). Antoine, K., et al. Mol. Cell. Biochem. 271 (1-2), 215-223 (2005): Grimwood, J., et al. Nature 428(6982):529-535(2004). Ferbus, D., et al. J. Pathol. 200(2):177-182(2003). Pibouin, L., et al. Cytogenet. Cell Genet. 92 (1-2), 80-84 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
zinc finger protein OZF
NCBI Official Synonym Full Names
zinc finger protein 146
NCBI Official Symbol
ZNF146
NCBI Official Synonym Symbols
OZF
NCBI Protein Information
zinc finger protein OZF
UniProt Protein Name
Zinc finger protein OZF
Protein Family
UniProt Gene Name
ZNF146
UniProt Synonym Gene Names
OZF
Uniprot Description
ZNF146: Belongs to the krueppel C2H2-type zinc-finger protein family.
Protein type: C2H2-type zinc finger protein; DNA-binding
Chromosomal Location of Human Ortholog: 19q13.12
Cellular Component: cytosol; nucleolus; nucleus
Molecular Function: DNA binding; DNA binding transcription factor activity; heparin binding; zinc ion binding
Biological Process: regulation of transcription, DNA-templated
Research Articles on ZNF146
Similar Products
Product Notes
The ZNF146 znf146 (Catalog #AAA6365681) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZNF146, NT (ZNF146, OZF, Zinc finger protein OZF, Only zinc finger protein, Zinc finger protein 146) (FITC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF146 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ZNF146 znf146 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZNF146, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
