Rabbit anti-Human Vitronectin Polyclonal Antibody | anti-VTN antibody

Anti-Vitronectin Antibody, Rabbit Polyclonal

Cat. Num. AAA8102753

• Gene Names: VTN; VN; V75; VNT
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A
• Synonyms: Vitronectin; Polyclonal Antibody; Anti-Vitronectin Antibody; Rabbit Polyclonal; Vitronectin Antibody; Rabbit PAb; vitronectin; Anti-V75 Antibody; Anti-VN Antibody; Anti-VNT Antibody; anti-VTN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Vitronectin
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-VTN antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:1000-1:2000
• Immunogen: Recombinant Human Vitronectin protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Vitronectin (rh VTN). Total IgG was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-vitronectin rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDaObserved band size:67 kDa)

Related Product Information for anti-VTN antibody

Vitronectin, also known as VTN, is a member of the pexin family. It is an abundant glycoprotein found in serum the extracellular matrix and promotes cell adhesion and spreading. Vitronectin is a secreted protein and exists in either a single chain form or a cleaved, two chain form held together by a disulfide bond. Vitronectin is a plasma glycoprotein implicated as a regulator of diverse physiological process, including blood coagulation, fibrinolysis, pericellular proteolysis, complement dependent immune responses, and cell attachment and spreading. Because of its ability to bind platelet glycoproteins and mediate platelet adhesion and aggregation at sites of vascular injury, vitronectin has become an important mediator in the pathogenesis of coronary atherosclerosis. As a multifunctional protein with a multiple binding domain, Vitronectin interacts with a variety of plasma and cell proteins. Vitronectin binds multiple ligands, including the soluble vitronectin receptor. It may be an independent predictor of adverse cardiovascular outcomes following acute stenting. Accordingly, Vitronectin is suggested to be involved in hemostasis, cell migration, as well as tumor malignancy.

References

Ekmeki OB, et al. (2006) Vitronectin in atherosclerotic disease. Clin Chim Acta. 368(1-2): 77-83.Derer W, et al. (2009) Vitronectin concentrations predict risk in patients undergoing coronary stenting. Circ Cardiovasc Interv. 2(1): 14-9.Heyman L, et al. (2010) Mesothelial vitronectin stimulates migration of ovarian cancer cells. Cell Biol Int. 34(5): 493-502.

NCBI and Uniprot Product Information

• NCBI GI #: 88853069
• NCBI GeneID: 7448
• NCBI Accession #: NP_000629.3
• NCBI GenBank Nucleotide #: NM_000638.3
• UniProt Accession #: P04004; P01141; Q9BSH7; B2R7G0
• Molecular Weight: 160,000
• NCBI Official Full Name: vitronectin
• NCBI Official Synonym Full Names: vitronectin
• NCBI Official Symbol: VTN
• NCBI Official Synonym Symbols: VN; V75; VNT
• NCBI Protein Information: vitronectin; epibolin; S-protein; somatomedin B; complement S-protein; serum spreading factor; serum-spreading factor
• UniProt Protein Name: Vitronectin
• Protein Family: Vitronectin
• UniProt Gene Name: VTN
• UniProt Synonym Gene Names: VN
• UniProt Entry Name: VTNC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008]

Uniprot Description

VTN: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 17q11

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: heparin binding; integrin binding; protein binding; extracellular matrix binding; scavenger receptor activity; polysaccharide binding

Biological Process: receptor-mediated endocytosis; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell-matrix adhesion; negative regulation of blood coagulation; positive regulation of smooth muscle cell migration; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of receptor-mediated endocytosis; positive regulation of peptidyl-tyrosine phosphorylation; regulation of complement activation; cell adhesion mediated by integrin; innate immune response; immune response; cell adhesion