Rabbit anti-Human ZN473 Polyclonal Antibody | anti-ZNF473 antibody

ZN473, NT (ZNF473, KIAA1141, ZFP100, Zinc finger protein 473, Zinc finger protein 100 homolog)

Cat. Num. AAA642003

• Gene Names: ZNF473; ZN473; HZFP100
• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ZN473; Polyclonal Antibody; NT (ZNF473; KIAA1141; ZFP100; Zinc finger protein 473; Zinc finger protein 100 homolog); Anti -ZN473; anti-ZNF473 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ZNF473 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Flow Cytometry: 1:10-50
• Immunogen: ZN473 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185~215 amino acids from the N-terminal region of human ZN473.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ZN473 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). ZN473 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(ZN473 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ZNF473 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 54792150
• NCBI GeneID: 25888
• NCBI Accession #: NP_001006657.1
• NCBI GenBank Nucleotide #: NM_001006656.1
• UniProt Accession #: Q8WTR7; Q9ULS9; Q9Y4Q7; A8K8T7
• Molecular Weight: 100,182 Da
• NCBI Official Full Name: zinc finger protein 473
• NCBI Official Synonym Full Names: zinc finger protein 473
• NCBI Official Symbol: ZNF473
• NCBI Official Synonym Symbols: ZN473; HZFP100
• NCBI Protein Information: zinc finger protein 473; zfp-100; zinc finger protein ZFP100; zinc finger protein 100 homolog
• UniProt Protein Name: Zinc finger protein 473
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZNF473
• UniProt Synonym Gene Names: KIAA1141; ZFP100; Zfp-100
• UniProt Entry Name: ZN473_HUMAN

Uniprot Description

ZNF473: Involved in histone 3'-end pre-mRNA processing by associating with U7 snRNP and interacting with SLBP/pre-mRNA complex. Increases histone 3'-end pre-mRNA processing but has no effect on U7 snRNP levels, when overexpressed. Required for cell cycle progression from G1 to S phases. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: nucleoplasm; Cajal body

Molecular Function: protein binding; DNA binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; histone mRNA metabolic process; histone mRNA 3'-end processing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription