Goat anti-Mouse Zinc Finger E-box Binding Homeobox 1 Polyclonal Antibody | anti-ZEB1 antibody

Zinc Finger E-box Binding Homeobox 1 (ZEB1, AREB6, BZP, NIL-2A, NIL-2-A, TCF8, ZEB, ZFHEP, ZFHX1A)

Cat. Num. AAA622171

• Gene Names: ZEB1; BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1; MGC133261
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Zinc Finger E-box Binding Homeobox 1; Polyclonal Antibody; Zinc Finger E-box Binding Homeobox 1 (ZEB1; AREB6; BZP; NIL-2A; NIL-2-A; TCF8; ZEB; ZFHEP; ZFHX1A); Anti -Zinc Finger E-box Binding Homeobox 1 (ZEB1; anti-ZEB1 antibody

• Host: Goat
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ZEB1 ar ~120kD. Species sequence homology: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-ZEB1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.
• Immunogen: Synthetic peptide, C-RPQIRQKIENKPLQE, from the internal region of human ZEB1.
• Positive Control: Human Heart and Mouse Heart lysates
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 monthsat -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Chemiluminescence

(ZEB1 (0.05ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-ZEB1 antibody

ZEB1 inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. Posterior polymorphous corneal dystrophy (PPCD) is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.

Product Categories/Family for anti-ZEB1 antibody

Antibodies; Abs to Homeobox (HOX)

NCBI and Uniprot Product Information

• NCBI GI #: 55957123
• NCBI GeneID: 6935
• UniProt Accession #: P37275; Q12924; Q13800; Q5T968; Q13088; Q2KJ05; Q32Q63; Q5T967; Q5VZ84; Q8NB68
• Molecular Weight: 124,074 Da
• NCBI Official Full Name: zinc finger E-box binding homeobox 1
• NCBI Official Synonym Full Names: zinc finger E-box binding homeobox 1
• NCBI Official Symbol: ZEB1
• NCBI Official Synonym Symbols: BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1; MGC133261
• NCBI Protein Information: zinc finger E-box-binding homeobox 1; OTTHUMP00000019404; OTTHUMP00000019405; OTTHUMP00000019406; negative regulator of IL2; delta-crystallin enhancer binding factor 1; posterior polymorphous corneal dystrophy 3; zinc finger homeodomain enhancer-binding protein; transcription factor 8 (represses interleukin 2 expression)
• UniProt Protein Name: Zinc finger E-box-binding homeobox 1
• Protein Family: Zinc finger E-box-binding homeobox
• UniProt Gene Name: ZEB1
• UniProt Synonym Gene Names: AREB6; TCF8
• UniProt Entry Name: ZEB1_HUMAN

NCBI Description

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.

Uniprot Description

TCF8: Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Interacts (via N-terminus) with SMARCA4/BRG1. Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas. Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Protein type: Motility/polarity/chemotaxis; Transcription, coactivator/corepressor; C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; double-stranded DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription corepressor activity; transcription factor activity

Biological Process: response to nutrient levels; transcription, DNA-dependent; regulation of T cell differentiation in the thymus; semicircular canal morphogenesis; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; embryonic skeletal morphogenesis; regulation of transcription from RNA polymerase II promoter; regulation of mesenchymal cell proliferation; cell proliferation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; cartilage development; forebrain development; regulation of smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; immune response; embryonic camera-type eye morphogenesis; response to activity; positive regulation of neuron differentiation; negative regulation of transcription, DNA-dependent

Disease: Corneal Dystrophy, Posterior Polymorphous, 3; Corneal Dystrophy, Fuchs Endothelial, 6