Selenoprotein W1 (SEPW1) Recombinant Protein | SEPW1 recombinant protein

Recombinant Selenoprotein W1 (SEPW1)

Cat. Num. AAA2011136

• Gene Names: SEPW1; selW
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Selenoprotein W1 (SEPW1); Recombinant Selenoprotein W1 (SEPW1); SEPW1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-MALAVRVVYC GAUGYKSKYL QLKKKLEDEF PGRLDICGEG TPQATGFFEV MVAGKLIHSK KKGDGYVDTE SKFLKLVAAI KAALAQG
• Sequence Length: 87

• Applicable Applications for SEPW1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Met1~Gly86 (Accession # P63302) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4506887
• NCBI GeneID: 6415
• NCBI Accession #: NP_003000.1
• NCBI GenBank Nucleotide #: NM_003009.2
• UniProt Accession #: P63302; O15532; O19096; Q86TI9; Q96KM5; A8MRR7
• Molecular Weight: 11.0kDa
• NCBI Official Full Name: selenoprotein W
• NCBI Official Synonym Full Names: selenoprotein W, 1
• NCBI Official Symbol: SEPW1
• NCBI Official Synonym Symbols: selW
• NCBI Protein Information: selenoprotein W
• UniProt Protein Name: Selenoprotein W
• Protein Family: Selenoprotein
• UniProt Gene Name: SEPW1
• UniProt Synonym Gene Names: SELW; SelW
• UniProt Entry Name: SELW_HUMAN

NCBI Description

This gene encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein shows highest expression in skeletal muscle and heart, and may be involved in oxidation-reduction reactions. A retroprocessed pseudogene, SEPW1P, has been identified and mapped to chromosome 1p35-34. [provided by RefSeq, Jul 2008]

Uniprot Description

SEPW1: Plays a role as a glutathione (GSH)-dependent antioxidant. May be involved in a redox-related process. May play a role in the myopathies of selenium deficiency. Belongs to the SelWTH family. SELW subfamily.

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: cytoplasm

Molecular Function: antioxidant activity; selenium binding

Biological Process: cell redox homeostasis