Western Blot (WB)
(Western blot analysis of lysates from mouse spleen, mouse thymus tissue lysate (from left to right), using Zcchc11 Antibody (C-term). MBS9214563 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Rabbit anti-Mouse Zcchc11 Polyclonal Antibody | anti-Zcchc11 antibody
(Mouse) Zcchc11 Antibody (C-term)
Gene Names
Zcchc11; PPAPD3; mKIAA0191; 6030404K05Rik; 9230115F04Rik
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Zcchc11; Polyclonal Antibody; (Mouse) Zcchc11 Antibody (C-term); Terminal uridylyltransferase 4; TUTase 4; Zinc finger CCHC domain-containing protein 11; Kiaa0191; Tut4; anti-Zcchc11 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This (Mouse) Zcchc11 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1568-1600 amino acids from the C-terminal region of (Mouse) Zcchc11.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
1,644
Applicable Applications for anti-Zcchc11 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Source
HUMAN
Cellular Location
Nucleus. Cytoplasm. Note=Translocates into the cytoplasm following treatment of the cell with LPS.
Tissue Location
Ubiquitously expressed.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysates from mouse spleen, mouse thymus tissue lysate (from left to right), using Zcchc11 Antibody (C-term). MBS9214563 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from mouse spleen, mouse thymus tissue lysate (from left to right), using Zcchc11 Antibody (C-term). MBS9214563 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Related Product Information for anti-Zcchc11 antibody
Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre- let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B (By similarity). Also catalyzes the 3' uridylation of miR- 26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation (By similarity).
References
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Okazaki N.,et al.Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
Carninci P.,et al.Science 309:1559-1563(2005).
Trost M.,et al.Immunity 30:143-154(2009).
Jones M.R.,et al.Nat. Cell Biol. 11:1157-1163(2009).
Okazaki N.,et al.Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
Carninci P.,et al.Science 309:1559-1563(2005).
Trost M.,et al.Immunity 30:143-154(2009).
Jones M.R.,et al.Nat. Cell Biol. 11:1157-1163(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
184650
NCBI Official Full Name
terminal uridylyltransferase 4
NCBI Official Synonym Full Names
zinc finger, CCHC domain containing 11
NCBI Official Symbol
Zcchc11
NCBI Official Synonym Symbols
PPAPD3; mKIAA0191; 6030404K05Rik; 9230115F04Rik
NCBI Protein Information
terminal uridylyltransferase 4
UniProt Protein Name
Terminal uridylyltransferase 4
Protein Family
UniProt Gene Name
Zcchc11
UniProt Synonym Gene Names
Kiaa0191; Tut4; TUTase 4
UniProt Entry Name
TUT4_MOUSE
Uniprot Description
ZCCHC11: a zinc finger protein that may participate in toll-receptor signaling by suppressing TRAF6-dependent activation of NF-kappaB. Two alternatively spliced isoforms have been reported.
Protein type: Cell development/differentiation; Transferase; RNA processing; EC 2.7.7.52
Cellular Component: extracellular space; cytoplasm; nucleolus; nucleus
Molecular Function: transferase activity; protein binding; nucleic acid binding; zinc ion binding; metal ion binding; RNA uridylyltransferase activity; nucleotidyltransferase activity
Biological Process: inhibition of NF-kappaB transcription factor; pre-microRNA processing; stem cell maintenance; cytokine production; positive regulation of interleukin-6 production; RNA-mediated gene silencing; regulation of lipopolysaccharide-mediated signaling pathway; RNA 3'-end processing
Research Articles on Zcchc11
Similar Products
Product Notes
The Zcchc11 zcchc11 (Catalog #AAA9214563) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The (Mouse) Zcchc11 Antibody (C-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Zcchc11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Zcchc11 zcchc11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Zcchc11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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