Rabbit anti-Mouse ULK3 Polyclonal Antibody | anti-ULK3 antibody

ULK3, CT (ULK3, Serine/threonine-protein kinase ULK3, Unc-51-like kinase 3) (AP)

Cat. Num. AAA6361388

• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ULK3; Polyclonal Antibody; CT (ULK3; Serine/threonine-protein kinase ULK3; Unc-51-like kinase 3) (AP); Unc-51-like kinase 3; anti-ULK3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ULK3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ULK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the C-terminal region of human ULK3.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ULK3 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ULK3 antibody detected the ULK3 protein (arrow).)

Related Product Information for anti-ULK3 antibody

Serine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. May exert this function by promoting GLI1 nuclear localization. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.

Product Categories/Family for anti-ULK3 antibody

Antibodies; Protein Kinases

References

Maloverjan, A., et al. J. Biol. Chem. 285(39):30079-30090(2010). Takeuchi, F., et al. Circulation 121(21):2302-2309(2010). Maloverjan, A., et al. Exp. Cell Res. 316(4):627-637(2010). Levy, D., et al. Nat. Genet. 41(6):677-687(2009). Kim, D.H., et al. Blood 113(11):2517-2525(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 150456432
• NCBI GeneID: 25989
• NCBI Accession #: NP_001092906.1
• NCBI GenBank Nucleotide #: NM_001099436.1
• UniProt Accession #: Q6PHR2; Q9NPN5; Q9UFS4; B2RXK3; B4DRQ7; D3DW68
• Molecular Weight: 53,444 Da
• NCBI Official Full Name: serine/threonine-protein kinase ULK3
• NCBI Official Synonym Full Names: unc-51-like kinase 3 (C. elegans)
• NCBI Official Symbol: ULK3
• NCBI Protein Information: serine/threonine-protein kinase ULK3
• UniProt Protein Name: Serine/threonine-protein kinase ULK3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: ULK3
• UniProt Entry Name: ULK3_HUMAN

Uniprot Description

ULK3: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; Other group; ULK family

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: negative regulation of smoothened signaling pathway; protein amino acid autophosphorylation; positive regulation of smoothened signaling pathway; autophagy; regulation of autophagy; autophagic vacuole formation