Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tubulin polymerization-promoting protein (TPPP) Standard Curve.)
Human Tubulin Polymerization-Promoting Protein (TPPP) ELISA Kit | TPPP elisa kit
Human Tubulin Polymerization-Promoting Protein (TPPP) ELISA Kit
Gene Names
TPPP; p24; p25; TPPP1; TPPP/p25; p25alpha
Reactivity
Human
Synonyms
Tubulin Polymerization-Promoting Protein (TPPP); Human Tubulin Polymerization-Promoting Protein (TPPP) ELISA Kit; TPPP; TPPP/p25; TPPP1; p24; p25; p25alpha; brain specific protein p25 alpha; glycogen synthase kinase 3 (GSK3) inhibitor p24; tubulin polymerization-promoting protein; TPPP elisa kit
Reactivity
Human
Specificity
Human Tubulin polymerization-promoting protein (TPPP) ELISA Kit has high sensitivity and excellent specificity for detection of Human TPPP. No significant cross-reactivity or interference between Human TPPP and analogues was observed.
Sequence Length
219
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tubulin polymerization-promoting protein (TPPP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tubulin polymerization-promoting protein (TPPP) Standard Curve.)
Related Product Information for TPPP elisa kit
Description: This Human Tubulin polymerization-promoting protein (TPPP) ELISA Kit employs a two-site sandwich ELISA to quantitate TPPP in samples. An antibody specific for TPPP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPPP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPPP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPPP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Seki et al. (1999) cloned p25 from a neuroblastoma cDNA library. The deduced 219-amino acid protein has a calculated molecular mass of about 24 kD. Human and bovine p25 share 90% amino acid identity. In contrast to the brain-specific expression of bovine p25, RT-PCR detected human p25 in all tissues examined.Martin et al. (2002) found that p24 associated with Gsk3 in microtubules from adult rat brain. p24 bound Gsk3 and inhibited its kinase activity at low magnesium concentrations. p24 was a poor substrate for Gsk3, but it could be phosphorylated by other protein kinases. Bovine Tppp inhibited mitotic spindle assembly and nuclear envelope breakdown following injection into Drosophila embryos. It did not affect other cellular events.
Background: Seki et al. (1999) cloned p25 from a neuroblastoma cDNA library. The deduced 219-amino acid protein has a calculated molecular mass of about 24 kD. Human and bovine p25 share 90% amino acid identity. In contrast to the brain-specific expression of bovine p25, RT-PCR detected human p25 in all tissues examined.Martin et al. (2002) found that p24 associated with Gsk3 in microtubules from adult rat brain. p24 bound Gsk3 and inhibited its kinase activity at low magnesium concentrations. p24 was a poor substrate for Gsk3, but it could be phosphorylated by other protein kinases. Bovine Tppp inhibited mitotic spindle assembly and nuclear envelope breakdown following injection into Drosophila embryos. It did not affect other cellular events.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tubulin polymerization-promoting protein
NCBI Official Synonym Full Names
tubulin polymerization promoting protein
NCBI Official Symbol
TPPP
NCBI Official Synonym Symbols
p24; p25; TPPP1; TPPP/p25; p25alpha
NCBI Protein Information
tubulin polymerization-promoting protein
UniProt Protein Name
Tubulin polymerization-promoting protein
Protein Family
UniProt Gene Name
TPPP
UniProt Synonym Gene Names
TPPP1; TPPP
UniProt Entry Name
TPPP_HUMAN
Uniprot Description
TPPP: May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown. Belongs to the TPPP family.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 5p15.3
Cellular Component: microtubule; perinuclear region of cytoplasm; cytoplasm; nucleus
Molecular Function: tubulin binding; protein binding; microtubule binding
Biological Process: positive regulation of protein polymerization; positive regulation of protein complex assembly; microtubule bundle formation; microtubule polymerization
Research Articles on TPPP
Similar Products
Product Notes
The Human TPPP tppp (Catalog #AAA9713812) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713812 ELISA Kit recognizes Human TPPP. It is sometimes possible for the material contained within the vial of "Tubulin Polymerization-Promoting Protein (TPPP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
