Rabbit ZAP 70 Polyclonal Antibody | anti-ZAP70 antibody

Rabbit anti ZAP 70

Cat. Num. AAA225179

• Gene Names: ZAP70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
• Reactivity: Human, Mouse, Rat (Expected from Sequence)
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified whole serum by affinity chromatography.
• Synonyms: ZAP 70; Polyclonal Antibody; Rabbit anti ZAP 70; ZAP 70 Antibody; anti-ZAP70 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified whole serum by affinity chromatography.
• Form/Format: Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
• Sequence Length: 619

• Applicable Applications for anti-ZAP70 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-Paraffin: 1:50-1:200; WB: 1:500-1:2000
• Immunogen: Recombinant human ZAP 70
• Preperation: Antiserum to ZAP 70 was raised by repeated immunization of rabbits with highly purified antigen.
• Preparation and Storage: Store at -20 degree C.; Storage in frost-free freezers is not recommended. This product should be stored undiluted.; Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blot analysis of cell and tissue lysates using Rabbit anti ZAP 70 antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human amygdalitis using Rabbit anti ZAP 70 antibody at a dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human amygdalitis using Rabbit anti ZAP 70 antibody at a dilution of 1/100)

Related Product Information for anti-ZAP70 antibody

Rabbit anti ZAP 70 antibody recognizes tyrosine-protein kinase ZAP 70 also known as Syk-related tyrosine kinase and 70 kDa zeta-chain associated protein. ZAP 70 belongs to the Sky/ZAP-70 protein kinase family and is involved in the regulation of the adaptive immune response.

NCBI and Uniprot Product Information

• NCBI GI #: 1177044
• NCBI GeneID: 7535
• UniProt Accession #: P43403; Q6PIA4; Q8IXD6; Q9UBS6; A6NFP4
• Molecular Weight: 55,873 Da
• NCBI Official Full Name: Tyrosine-protein kinase ZAP-70
• NCBI Official Synonym Full Names: zeta chain of T cell receptor associated protein kinase 70
• NCBI Official Symbol: ZAP70
• NCBI Official Synonym Symbols: SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
• NCBI Protein Information: tyrosine-protein kinase ZAP-70
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ZAP70
• UniProt Synonym Gene Names: SRK

NCBI Description

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).