Mouse anti-Human ZAP70 Monoclonal Antibody | anti-ZAP70 antibody

Anti-ZAP70 Antibody

Cat. Num. AAA418414

• Gene Names: ZAP70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified by protein A affinity chromatography
• Synonyms: ZAP70; Monoclonal Antibody; Anti-ZAP70 Antibody; anti-ZAP70 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: Purified by protein A affinity chromatography
• Form/Format: Liquid
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 493

• Applicable Applications for anti-ZAP70 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Ni-NTA purified truncated recombinant ZAP70 expressed in E Coli strain BL21 (DE3)
• Storage Buffer: PBS, pH 7.4 with 0.05% sodium azide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Immunohistochemistry (IHC)

Related Product Information for anti-ZAP70 antibody

ZAP70 (zeta-chain associated protein kinase), a 70 kDa member of the SYK tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Zap70 controls TCR (T-cell antigen receptor)-linked signal transduction pathways. Its key role in thymocytes development and mature T lymphocytes activation has been illustrated by the characterization of several human immunodeficiencies presenting with mutations in the zap-70 gene. ZAP-70 is also expressed in several types of B-cell neoplasm and is easily detected by immunohistochemistry, providing a useful prognostic marker in patients with chronic lymphocytic leukaemia.

References

1.Sigal Gelkop, Gerrald D. Gish, Yael Babichev J Immunol. 2005 Dec 15;175 (12):8123-32 2.Joaquim Carreras, Neus Villamor, Lluis Colomo J Pathol. 2005 Mar;205 (4):507-13 3.Claire Hivroz Med Sci (Paris). 2005 Feb;21 (2):150-5

NCBI and Uniprot Product Information

• NCBI GI #: 24657846
• NCBI GeneID: 7535
• Molecular Weight: 55,873 Da
• NCBI Official Full Name: ZAP70 protein
• NCBI Official Synonym Full Names: zeta chain of T-cell receptor associated protein kinase 70
• NCBI Official Symbol: ZAP70
• NCBI Official Synonym Symbols: SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
• NCBI Protein Information: tyrosine-protein kinase ZAP-70
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ZAP70
• UniProt Synonym Gene Names: SRK

NCBI Description

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ZAP70: a tyrosine kinase of the Syk family. Plays an essential role in regulation of the adaptive immune response. Phosphorylates two essential T cell adapter proteins: the linker for activation of T-cells (LAT) protein and LCP2. Regulates lymphocyte activation, motility, adhesion and cytokine expression of mature T-cells. Promotes survival and cell-cycle progression of developing double-negative thymocytes before positive selection. Associates with the T-cell antigen receptor (TCR) zeta-chain and is phosphorylated by Lck following antigen receptor activation. Required for internalization and degradation of the TCR complex through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Three alternatively spliced isoforms of the human protein have been described.

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Syk family; TK group

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; lipid raft; plasma membrane; T cell receptor complex

Molecular Function: non-membrane spanning protein tyrosine kinase activity; protein binding; protein-tyrosine kinase activity; receptor binding

Biological Process: adaptive immune response; B cell activation; B cell receptor signaling pathway; immune response; inflammatory response; innate immune response; macrophage activation during immune response; neutrophil activation during immune response; peptidyl-tyrosine phosphorylation; positive regulation of alpha-beta T cell proliferation; positive regulation of B cell differentiation; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; positive regulation of T cell differentiation; positive thymic T cell selection; protein amino acid phosphorylation; T cell activation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Autoimmune Disease, Multisystem, Infantile-onset, 2; Selective T-cell Defect