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Western Blot (WB) (Western Blot analysis of CD69 polyclonal antibody)

Rabbit anti-Human, Mouse CD69 Polyclonal Antibody | anti-CD69 antibody

CD69 Polyclonal Antibody

Gene Names
CD69; AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
Reactivity
Human, Mouse
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
CD69; Polyclonal Antibody; CD69 Polyclonal Antibody; Early activation antigen CD69; Activation inducer molecule; AIM; BL-AC/P26; C-type lectin domain family 2 member C; EA1; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3; CD_antigen=CD69; CLEC2C; anti-CD69 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CD69 polyclonal antibody detects endogenous levels of CD69 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
199
Applicable Applications for anti-CD69 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant full length Human CD69.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of CD69 polyclonal antibody)

Western Blot (WB) (Western Blot analysis of CD69 polyclonal antibody)
Related Product Information for anti-CD69 antibody
CD69 is a disulfide linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. It is a C type lectin, most closely related to the NKRP1 and Ly49 NK cell activation molecules. CD69 is involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. It is the earliest inducible cell surface glycoprotein acquired during lymphoid activation. Induction occurs by antigens, mitogens or activators of PKC on the surface of T and B lymphocytes, and by interaction of IL2 with the p75 IL2R on the surface of NK cells. Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
969
UniProt Accession #
Molecular Weight
~ 25kDa
NCBI Official Full Name
Early activation antigen CD69
NCBI Official Synonym Full Names
CD69 molecule
NCBI Official Symbol
CD69
NCBI Official Synonym Symbols
AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
NCBI Protein Information
early activation antigen CD69
UniProt Protein Name
Early activation antigen CD69
Protein Family
UniProt Gene Name
CD69
UniProt Synonym Gene Names
CLEC2C; AIM
UniProt Entry Name
CD69_HUMAN

NCBI Description

This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]

Uniprot Description

Function: Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

Subunit structure: Homodimer; disulfide-linked. Ref.6 Ref.7 Ref.8

Subcellular location: Membrane; Single-pass type II membrane protein.

Tissue specificity: Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.

Developmental stage: Earliest inducible cell surface glycoprotein acquired during lymphoid activation.

Induction: By antigens, mitogens or activators of PKC on the surface of T and B-lymphocytes. By interaction of IL-2 with the p75 IL-2R on the surface of NK cells.

Post-translational modification: Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.

Sequence similarities: Contains 1 C-type lectin domain.

Research Articles on CD69

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Product Notes

The CD69 cd69 (Catalog #AAA3008035) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD69 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD69 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the CD69 cd69 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD69, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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