Rabbit ZAK Polyclonal Antibody | anti-ZAK antibody

ZAK Polyclonal Antibody

Cat. Num. AAA3012075

• Gene Names: MAP3K20; pk; AZK; MLT; MRK; ZAK; CNM6; MLK7; MLTK; SFMMP; mlklak; MLTKbeta; MLTKalpha
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ZAK; Polyclonal Antibody; ZAK Polyclonal Antibody; Mitogen-activated protein kinase kinase kinase MLT; Human cervical cancer suppressor gene 4 protein; HCCS-4; Leucine zipper- and sterile alpha motif-containing kinase; MLK-like mitogen-activated protein triple kinase; Mixed lineage kinase-related kinase; MLK-related kinase; MRK; Sterile alpha motif- and leucine zipper-containing kinase AZK; MLTK; HCCS4; anti-ZAK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ZAK polyclonal antibody detects endogenous levels of ZAK protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 800

• Applicable Applications for anti-ZAK antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:20-1:50
• Immunogen: Recombinant full length Human ZAK.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using ZAK antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon carcinoma using ZAK antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells using ZAK antibody.)

Related Product Information for anti-ZAK antibody

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

NCBI and Uniprot Product Information

• NCBI GI #: 82880648
• NCBI GeneID: 51776
• NCBI Accession #: NP_057737.2
• NCBI GenBank Nucleotide #: NP_057737.2
• UniProt Accession #: Q9NYL2
• Molecular Weight: ~ 55, 91, 100kDa
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 20 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 20
• NCBI Official Symbol: MAP3K20
• NCBI Official Synonym Symbols: pk; AZK; MLT; MRK; ZAK; CNM6; MLK7; MLTK; SFMMP; mlklak; MLTKbeta; MLTKalpha
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 20
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase MLT
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MLTK
• UniProt Synonym Gene Names: ZAK; HCCS-4; MLK-related kinase; MRK
• UniProt Entry Name: MLTK_HUMAN

NCBI Description

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ZAK: a mixed-lineage kinase containing a leucine-zipper (LZ) and a sterile-alpha motif (SAM). It is reported to activate the JNK/SAPK and NF-kappaB pathways.

Protein type: EC 2.7.11.25; Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; MLK subfamily

Chromosomal Location of Human Ortholog: 2q24.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding

Biological Process: cell death; cell proliferation; response to radiation; activation of MAPKK activity; positive regulation of apoptosis; DNA damage checkpoint; response to stress; cytoskeleton organization and biogenesis; cell cycle arrest; cell differentiation; protein amino acid phosphorylation; cell cycle checkpoint; activation of JNK activity