Mouse Kallikrein 8, KLK8 ELISA Kit | KLK8 elisa kit

Mouse Kallikrein 8, KLK8 ELISA Kit

Cat. Num. AAA1600931

• Gene Names: KLK8; NP; HNP; NRPN; PRSS19; TADG14
• Reactivity: Mouse
• Synonyms: Kallikrein 8; KLK8; Mouse Kallikrein 8; KLK8 ELISA Kit; KLK8 elisa kit

• Reactivity: Mouse
• Sequence Length: 139

• Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05ng/ml-10ng/ml
• Sensitivity: 0.025ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLK8 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse insulin autoantibodies (also known as IAA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse IAA antibody. IAA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse IAA Antibody is added and binds to IAA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IAA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse IAA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 527121930
• NCBI GeneID: 11202
• NCBI Accession #: NP_001268360.1
• NCBI GenBank Nucleotide #: NM_001281431.1
• UniProt Accession #: O60259; Q5V9X1; Q5V9X2; Q8IW69; Q9HCB3; Q9NR68; Q9NR69; Q9UIL9; Q9UQ47
• Molecular Weight: 32,966 Da
• NCBI Official Full Name: kallikrein-8 isoform 5
• NCBI Official Synonym Full Names: kallikrein related peptidase 8
• NCBI Official Symbol: KLK8
• NCBI Official Synonym Symbols: NP; HNP; NRPN; PRSS19; TADG14
• NCBI Protein Information: kallikrein-8
• UniProt Protein Name: Kallikrein-8
• Protein Family: Kallikrein
• UniProt Gene Name: KLK8
• UniProt Synonym Gene Names: NRPN; PRSS19; TADG14; hK8; NP

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in tandem in a gene cluster on chromosome 19. The encoded protein may be involved in proteolytic cascade in the skin and may serve as a biomarker for ovarian cancer. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury.