Rabbit YAP / YAP1 Polyclonal Antibody | anti-YAP / YAP1 antibody

Anti-YAP / YAP1 Antibody (aa93-142) IHC-plus

Cat. Num. AAA2400148

• Gene Names: YAP1; YAP; YKI; COB1; YAP2; YAP65
• Reactivity: Mouse, Rat, Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity purified
• Synonyms: YAP / YAP1; Polyclonal Antibody; Anti-YAP / YAP1 Antibody (aa93-142) IHC-plus; Rabbit Polyclonal (IgG) to Human YAP / YAP1; Human YAP / YAP1; YAP1; 65 kDa Yes-associated protein; YAP; Yes-associated protein beta; Yes-associated protein delta; Yorkie homolog; YAP2; Yes-associated protein 1; Yes-associated protein 2; YKI; YAP65; anti-YAP / YAP1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: YAP Antibody detects endogenous levels of total YAP protein.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 450

• Applicable Applications for anti-YAP / YAP1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:1000); IHC-P (20 ug/ml); WB (1:500 - 1:1000)
• Immunogen: YAP / YAP1 antibody was raised against synthetic peptide from human YAP (aa93-142).
• Immunogen Description: Synthetic peptide from human YAP (aa93-142).
• Antigen Modification: aa93-142
• Target Species: Human
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-YAP / YAP1 antibody IHC staining of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(Western blot of extracts from HepG2 cells, treated with Wortmannin 40 nM 24h, using YAP (Ab-127) Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-YAP / YAP1 antibody

YAP1 Antibody, 65 kDa Yes-associated protein Antibody, YAP Antibody, Yes-associated protein beta Antibody, Yes-associated protein delta Antibody, Yorkie homolog Antibody, YAP2 Antibody, Yes-associated protein 1 Antibody, Yes-associated protein 2 Antibody, YKI Antibody, YAP65 Antibody Description: Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 303523511
• NCBI GeneID: 10413
• NCBI Accession #: NP_006097.2
• NCBI GenBank Nucleotide #: NM_006106.4
• UniProt Accession #: P46937; Q7Z574; B4DTY1; B7ZA01; E3WEB5; E3WEB6; E9PRV2; F5H202; K0KQ18; K0KYZ8; K0L195; K0L1G3
• Molecular Weight: 54,942 Da
• NCBI Official Full Name: transcriptional coactivator YAP1 isoform 2
• NCBI Official Synonym Full Names: Yes associated protein 1
• NCBI Official Symbol: YAP1
• NCBI Official Synonym Symbols: YAP; YKI; COB1; YAP2; YAP65
• NCBI Protein Information: transcriptional coactivator YAP1
• UniProt Protein Name: Transcriptional coactivator YAP1
• UniProt Gene Name: YAP1
• UniProt Synonym Gene Names: YAP65; Yes-associated protein 1
• UniProt Entry Name: YAP1_HUMAN

NCBI Description

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]

Uniprot Description

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288). The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750).