Rabbit Phospho-YAP (S127) Polyclonal Antibody | anti-YAP1 antibody

Phospho-YAP (S127) Antibody

Cat. Num. AAA9201689

• Gene Names: YAP1; YAP; YKI; COB1; YAP2; YAP65
• Reactivity: Human (Predicted Reactivity: Chicken, Mouse, Rat)
• Applications: Dot Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Phospho-YAP (S127); Polyclonal Antibody; Phospho-YAP (S127) Antibody; Transcriptional coactivator YAP1; Yes-associated protein 1; Protein yorkie homolog; Yes-associated protein YAP65 homolog; YAP1; YAP65; anti-YAP1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Chicken, Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This YAP Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S127 of human YAP.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 504

• Applicable Applications for anti-YAP1 antibody: Dot Blot (DB), ELISA (EIA)
• Application Notes: DB~~1:500
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Dot Blot (DB)

(Dot blot analysis of Phospho-YAP-S127 antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-YAP1 antibody

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).

Product Categories/Family for anti-YAP1 antibody

Cardiovascular; Autophagy

NCBI and Uniprot Product Information

• NCBI GI #: 194306653
• NCBI GeneID: 10413
• NCBI Accession #: NP_001123617.1; NP_001181973.1; NP_001181974.1; NP_001269027.1; NP_001269028.1; NP_001269029.1; NP_001269030.1
• NCBI GenBank Nucleotide #: NM_001130145.2
• UniProt Accession #: P46937; Q7Z574; B4DTY1; B7ZA01; E3WEB5; E3WEB6; E9PRV2; F5H202; K0KQ18; K0KYZ8; K0L195; K0L1G3
• Molecular Weight: 54462
• NCBI Official Full Name: yorkie homolog isoform 1
• NCBI Official Synonym Full Names: Yes-associated protein 1
• NCBI Official Symbol: YAP1
• NCBI Official Synonym Symbols: YAP; YKI; COB1; YAP2; YAP65
• NCBI Protein Information: transcriptional coactivator YAP1
• UniProt Protein Name: Transcriptional coactivator YAP1
• Protein Family: Transcriptional coactivator
• UniProt Gene Name: YAP1
• UniProt Synonym Gene Names: YAP65; Yes-associated protein 1
• UniProt Entry Name: YAP1_HUMAN

NCBI Description

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]

Uniprot Description

YAP1: an adaptor protein that binds to HER4 and the SH3 domain of the Yes tyrosine kinase. Associates with multiple transcription factors in the nucleus, and appears to be a co-transcriptional activator for the carboxyl-terminal fragment of ErbB-4 that translocates to the nucleus. Contains a WW domain that is found in various structural, regulatory and signaling molecules.

Protein type: Oncoprotein; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; transcription factor complex; membrane; cytoplasm; nucleus; cytosol; cell junction

Molecular Function: protein binding; transcription coactivator activity; chromatin binding; transcription corepressor activity

Biological Process: paraxial mesoderm development; transcription initiation from RNA polymerase II promoter; somatic stem cell maintenance; lateral mesoderm development; cell morphogenesis; negative regulation of epithelial cell differentiation; notochord development; positive regulation of organ growth; keratinocyte differentiation; cell proliferation; positive regulation of cell proliferation; gene expression; positive regulation of transcription from RNA polymerase II promoter; vasculogenesis; response to DNA damage stimulus

Disease: Coloboma, Ocular, With Or Without Hearing Impairment, Cleft Lip/palate, And/or Mental Retardation