Rabbit anti-Human XRN2 Polyclonal Antibody | anti-XRN2 antibody

XRN2 Rabbit pAb

Cat. Num. AAA9143274

• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: XRN2; Polyclonal Antibody; XRN2 Rabbit pAb; anti-XRN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-XRN2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant protein of human XRN2.
• Positive Samples: PC-3, A-549
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using XRN2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)

Related Product Information for anti-XRN2 antibody

Background: This gene encodes a 5'-3' exonuclease that promotes transcription termination at cotranscriptional cleavage sites. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Categories/Family for anti-XRN2 antibody

Epigenetics & Nuclear Signaling; RNA Binding

NCBI and Uniprot Product Information

• NCBI GI #: 18860916
• NCBI GeneID: 22803
• NCBI Accession #: NP_036387.2
• NCBI GenBank Nucleotide #: NM_012255.3
• UniProt Accession #: Q9H0D6; Q3L8N4; Q6KGZ9; Q9BQL1; Q9NTW0; Q9NXS6; Q9UL53
• Molecular Weight: 108,582 Da
• NCBI Official Full Name: 5'-3' exoribonuclease 2
• NCBI Official Synonym Full Names: 5'-3' exoribonuclease 2
• NCBI Official Symbol: XRN2
• NCBI Protein Information: 5'-3' exoribonuclease 2; DHP protein; Dhm1-like protein (mouse homolog)
• UniProt Protein Name: 5'-3' exoribonuclease 2
• UniProt Gene Name: XRN2
• UniProt Synonym Gene Names: DHP protein
• UniProt Entry Name: XRN2_HUMAN

NCBI Description

This gene shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The yeast gene is involved in homologous recombination and RNA metabolism, such as RNA synthesis and RNA trafficking. Complementation studies show that Dhm1 has a similar function in mouse as dhp1. The function of the human gene has not yet been determined. Transcript variants encoding different isoforms have been noted for this gene; however, their full-length nature is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

XRN2: Possesses 5'->3' exoribonuclease activity. May promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription. Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.13.-; Ribonuclease; RNA processing; RNA-binding; Cell development/differentiation; Nucleolus

Chromosomal Location of Human Ortholog: 20p11.2-p11.1

Cellular Component: membrane; nucleolus; nucleus

Molecular Function: 5'-3' exonuclease activity; 5'-3' exoribonuclease activity; nuclease activity; zinc ion binding

Biological Process: RNA processing; regulation of transcription, DNA-dependent; RNA metabolic process; RNA catabolic process; spermatogenesis; cell growth; mRNA processing; DNA catabolic process, exonucleolytic; transcription termination