Human Aquaporin 1, Colton Blood Group (AQP1) ELISA Kit | AQP1 elisa kit

Human Aquaporin 1, Colton Blood Group (AQP1) ELISA Kit

Cat. Num. AAA2019511

• Gene Names: AQP1; CO; CHIP28; AQP-CHIP
• Reactivity: Human
• Synonyms: Aquaporin 1; Colton Blood Group (AQP1); Human Aquaporin 1; Colton Blood Group (AQP1) ELISA Kit; AQP-CHIP; CHIP28; CO; Channel-Forming Integral Protein; 28kDa; Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule; AQP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Aquaporin 1, Colton Blood Group (AQP1).; No significant cross-reactivity or interference between Aquaporin 1, Colton Blood Group (AQP1) and analogues was observed.
• Sequence Length: 269

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.25-16ng/mL
• Sensitivity: < 0.09ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aquaporin 1, Colton Blood Group (AQP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aquaporin 1, Colton Blood Group (AQP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aquaporin 1, Colton Blood Group (AQP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aquaporin 1, Colton Blood Group (AQP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aquaporin 1, Colton Blood Group (AQP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aquaporin 1, Colton Blood Group (AQP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for AQP1 elisa kit

Hormone metabolism; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 37694062
• NCBI GeneID: 358
• NCBI Accession #: NP_932766.1
• NCBI GenBank Nucleotide #: NM_198098.2
• UniProt Accession #: P29972; Q8TBI5; Q8TDC1; B5BU39; E7EM69; E9PC21; F5GY19
• Molecular Weight: 16,677 Da
• NCBI Official Full Name: aquaporin-1 isoform 1
• NCBI Official Synonym Full Names: aquaporin 1 (Colton blood group)
• NCBI Official Symbol: AQP1
• NCBI Official Synonym Symbols: CO; CHIP28; AQP-CHIP
• NCBI Protein Information: aquaporin-1
• UniProt Protein Name: Aquaporin-1
• UniProt Gene Name: AQP1
• UniProt Synonym Gene Names: CHIP28; AQP-1
• UniProt Entry Name: AQP1_HUMAN

NCBI Description

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, aquaporin family

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: nuclear membrane; integral to plasma membrane; basolateral plasma membrane; brush border membrane; apical part of cell; apical plasma membrane; cytoplasm; plasma membrane; basal plasma membrane; nerve terminal; nucleus; brush border; sarcolemma

Molecular Function: protein binding; ephrin receptor binding; potassium channel activity; water transporter activity; intracellular cGMP activated cation channel activity; ammonium transmembrane transporter activity; potassium ion transmembrane transporter activity; glycerol transmembrane transporter activity; transmembrane transporter activity; nitric oxide transporter activity; water channel activity; glycerol channel activity

Biological Process: cGMP biosynthetic process; glycerol transport; cerebrospinal fluid secretion; wound healing; carbon dioxide transport; water transport; cellular response to stress; negative regulation of caspase activity; sensory perception of pain; pancreatic juice secretion; positive regulation of saliva secretion; odontogenesis; positive regulation of fibroblast proliferation; hyperosmotic salinity response; nitric oxide transport; bicarbonate transport; multicellular organismal water homeostasis; cellular homeostasis; transmembrane transport; lateral ventricle development; lipid digestion; potassium ion transport; response to drug; cellular water homeostasis; secretory granule organization and biogenesis; glomerular filtration; ammonium transport; renal water transport; positive regulation of angiogenesis; camera-type eye morphogenesis; adrenocorticotropin hormone secretion; response to estrogen stimulus; establishment and/or maintenance of actin cytoskeleton polarity; renal water homeostasis; cell volume homeostasis; negative regulation of apoptosis

Disease: Blood Group--colton