Rabbit anti-Human, Mouse XRCC6 / Ku70 Polyclonal Antibody | anti-XRCC6 antibody

Rabbit Polyclonal (IgG) to Human XRCC6 / Ku70

Cat. Num. AAA247726

• Gene Names: XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: XRCC6 / Ku70; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human XRCC6 / Ku70; Anti-XRCC6 / Ku70 Antibody (aa1-50) IHC-plus; XRCC6; 5-dRP lyase Ku70; 70 kDa subunit of Ku antigen; CTCBF; DNA repair protein XRCC6; D22S671; D22S731; G22P1; Ku autoantigen; 70kDa; Ku autoantigen p70 subunit; Thyroid-lupus autoantigen p70; TLAA; CTC75; KU70; ML8; Thyroid-lupus autoantigen; Human XRCC6; Ku70; anti-XRCC6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Ku70 Antibody detects endogenous levels of total Ku70 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 609

• Applicable Applications for anti-XRCC6 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human Ku70 (aa1-50).
• Immunogen: XRCC6 / Ku70 antibody was raised against synthetic peptide from human Ku70 (aa1-50).
• Antigen Modification: aa1-50
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-XRCC6 / Ku70 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of extracts from 293 cells, using Ku70 Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 4503841
• NCBI GeneID: 2547
• NCBI Accession #: NP_001460.1
• NCBI GenBank Nucleotide #: NM_001469.4
• UniProt Accession #: P12956; Q6FG89; Q9UCQ2; Q9UCQ3; B1AHC8
• Molecular Weight: 65,149 Da
• NCBI Official Full Name: X-ray repair cross-complementing protein 6 isoform 1
• NCBI Official Synonym Full Names: X-ray repair complementing defective repair in Chinese hamster cells 6
• NCBI Official Symbol: XRCC6
• NCBI Official Synonym Symbols: ML8; KU70; TLAA; CTC75; CTCBF; G22P1
• NCBI Protein Information: X-ray repair cross-complementing protein 6; 5'-dRP lyase Ku70; Ku autoantigen, 70kDa; DNA repair protein XRCC6; Ku autoantigen p70 subunit; 70 kDa subunit of Ku antigen; thyroid-lupus autoantigen p70; lupus Ku autoantigen protein p70; 5'-deoxyribose-5-phosphate lyase Ku70; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; thyroid autoantigen 70kD (Ku antigen); ATP-dependent DNA helicase 2 subunit 1; thyroid autoantigen 70kDa (Ku antigen); ATP-dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase II, 70 kDa subunit
• UniProt Protein Name: X-ray repair cross-complementing protein 6
• Protein Family: X-ray repair cross-complementing protein
• UniProt Gene Name: XRCC6
• UniProt Synonym Gene Names: G22P1; 5'-dRP lyase Ku70; CTC75; CTCBF; Ku70; TLAA
• UniProt Entry Name: XRCC6_HUMAN

NCBI Description

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Uniprot Description

Ku70: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.

Protein type: DNA-binding; Nuclear receptor co-regulator; EC 4.2.99.-; RNA-binding; Helicase; DNA repair, damage; EC 3.6.4.-

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; transcription factor complex; nuclear telomere cap complex; membrane; nucleolus; nucleus; cytosol

Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; DNA binding; double-stranded DNA binding; damaged DNA binding; double-stranded telomeric DNA binding; 5'-deoxyribose-5-phosphate lyase activity; ATP binding

Biological Process: positive regulation of neurogenesis; V(D)J recombination; transcription, DNA-dependent; viral reproduction; positive regulation of transcription, DNA-dependent; DNA repair; double-strand break repair via nonhomologous end joining; DNA duplex unwinding; DNA ligation; double-strand break repair; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transcription, DNA-dependent; telomere maintenance