Testing Data
anti-Human, Mouse CARD10 Antibody | anti-CARD10 antibody
CARD10 Antibody
Gene Names
CARD10; BIMP1; CARMA3
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
CARD10; CARD10 Antibody; Caspase recruitment domain-containing protein 10; CARD-containing MAGUK protein 3; Carma 3; CARMA3; anti-CARD10 antibody
Reactivity
Human, Mouse
Specificity
CARD10 antibody detects endogenous levels of total CARD10 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
1032
Applicable Applications for anti-CARD10 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:1000
IF: 1:100~1:500
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human CARD10.
Preparation and Storage
Store at-20 degree C for 1 year.
Testing Data
Testing Data
Western Blot (WB)
(Peptide-+ Western blot analysis of extracts from HeLa cells and 293 cells, using CARD10 antibody. Immunofluorescence analysis of HeLa cells, using CARD10 antibody.)
Western Blot (WB)
(Peptide-+ Western blot analysis of extracts from HeLa cells and 293 cells, using CARD10 antibody. Immunofluorescence analysis of HeLa cells, using CARD10 antibody.)
Product Categories/Family for anti-CARD10 antibody
References
Wang L., . Biol. Chem. 276:21405-21409(2001). Gaide O., FEBS Lett. 496:121-127(2001). Dunham I., Nature 402:489-495(1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
82,541 Da
NCBI Official Full Name
caspase recruitment domain-containing protein 10
NCBI Official Synonym Full Names
caspase recruitment domain family member 10
NCBI Official Symbol
CARD10
NCBI Official Synonym Symbols
BIMP1; CARMA3
NCBI Protein Information
caspase recruitment domain-containing protein 10
UniProt Protein Name
Caspase recruitment domain-containing protein 10
Protein Family
UniProt Gene Name
CARD10
UniProt Synonym Gene Names
CARMA3; Carma 3
NCBI Description
The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008]
Uniprot Description
Activates NF-kappa-B via BCL10 and IKK.CautionSupposed to contain a SH3, a PDZ and a guanylate kinase-like domain. But none of these 3 domains are detected by PROSITE, Pfam or SMART.
Research Articles on CARD10
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Product Notes
The CARD10 card10 (Catalog #AAA851997) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The CARD10 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CARD10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000. Researchers should empirically determine the suitability of the CARD10 card10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CARD10, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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