Rabbit anti-Human XRCC1 Polyclonal Antibody | anti-XRCC1 antibody

XRCC1, ID (XRCC1, DNA repair protein XRCC1, X-ray repair cross-complementing protein 1) (APC)

Cat. Num. AAA6364051

• Gene Names: XRCC1; RCC
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: XRCC1; Polyclonal Antibody; ID (XRCC1; DNA repair protein XRCC1; X-ray repair cross-complementing protein 1) (APC); X-ray repair cross-complementing protein 1; anti-XRCC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-XRCC1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IF: 1:10-50; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: XRCC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-443 amino acids from the Central region of human XRCC1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(XRCC1 Antibody (Center) western blot analysis in A375 cell line lysates (15ug/lane).This demonstrates the XRCC1 antibody detected the XRCC1 protein (arrow).)

Immunohistochemistry (IHC)

(XRCC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XRCC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(XRCC1 Antibody (Center) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of XRCC1 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-XRCC1 antibody

The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Product Categories/Family for anti-XRCC1 antibody

Antibodies; DNA Replication; Repair

References

Lamerdin, J.E., et al. Genomics 25(2):547-554(1995) Gyapay, G., et al. Nat. Genet. 7 (2 SPEC NO), 246-339 (1994): Thompson, L.H., et al. Mol. Cell. Biol. 10(12):6160-6171(1990) Thompson, L.H., et al. Genomics 5(4):670-679(1989).

NCBI and Uniprot Product Information

• NCBI GI #: 190684675
• NCBI GeneID: 7515
• NCBI Accession #: NP_006288.2
• NCBI GenBank Nucleotide #: NM_006297.2
• UniProt Accession #: P18887; Q6IBS4; Q9HCB1
• Molecular Weight: 69,477 Da
• NCBI Official Full Name: DNA repair protein XRCC1
• NCBI Official Synonym Full Names: X-ray repair complementing defective repair in Chinese hamster cells 1
• NCBI Official Symbol: XRCC1
• NCBI Official Synonym Symbols: RCC
• NCBI Protein Information: DNA repair protein XRCC1; X-ray repair cross-complementing protein 1; X-ray-repair, complementing defective, repair in Chinese hamster
• UniProt Protein Name: DNA repair protein XRCC1
• Protein Family: DNA repair protein
• UniProt Gene Name: XRCC1
• UniProt Entry Name: XRCC1_HUMAN

NCBI Description

The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]

Uniprot Description

XRCC1: Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF. Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; enzyme binding; damaged DNA binding

Biological Process: single strand break repair; base-excision repair; DNA repair