Rabbit anti-Human XPC Polyclonal Antibody | anti-XPC antibody

XPC, NT (XPC, XPCC, DNA repair protein complementing XP-C cells, Xeroderma pigmentosum group C-complementing protein, p125) (FITC)

Cat. Num. AAA6364009

• Gene Names: XPC; XP3; RAD4; XPCC; p125
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: XPC; Polyclonal Antibody; NT (XPC; XPCC; DNA repair protein complementing XP-C cells; Xeroderma pigmentosum group C-complementing protein; p125) (FITC); p125; anti-XPC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-XPC antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: XPC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the N-terminal region of human XPC.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(XPC Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the XPC antibody detected the XPC protein (arrow).)

Related Product Information for anti-XPC antibody

This gene encodes a component of the nucleotide excision repair (NER) pathway. There are multiple components involved in the NER pathway, including Xeroderma pigmentosum (XP) A-G and V, Cockayne syndrome (CS) A and B, and trichothiodystrophy (TTD) group A, etc. This component, XPC, plays an important role in the early steps of global genome NER, especially in damage recognition, open complex formation, and repair protein complex formation. Mutations in this gene or some other NER components result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene.

Product Categories/Family for anti-XPC antibody

Antibodies; DNA Replication; Repair

References

Gangwar, R., et al. J. Cancer Res. Clin. Oncol. (2009). In press: Agalliu, I., et al. Cancer Causes Control (2009). In press: Langie, S.A., et al. Br. J. Nutr., 1-12 (2009). In press: Young, R.P., et al. Postgrad Med J 85(1008):515-524(2009). Stern, M.C., et al. Cancer Res. 69(17):6857-6864(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 224809302
• NCBI GeneID: 7508
• NCBI Accession #: NP_001139241.1; NP_004619.3
• NCBI GenBank Nucleotide #: NM_001145769.1
• UniProt Accession #: Q01831; Q53GT7; Q96AX0; B4DIP3; E9PB96; E9PH69
• Molecular Weight: 15,712 Da
• NCBI Official Synonym Full Names: XPC complex subunit, DNA damage recognition and repair factor
• NCBI Official Symbol: XPC
• NCBI Official Synonym Symbols: XP3; RAD4; XPCC; p125
• NCBI Protein Information: DNA repair protein complementing XP-C cells
• UniProt Protein Name: DNA repair protein complementing XP-C cells
• Protein Family: DNA repair protein
• UniProt Gene Name: XPC
• UniProt Synonym Gene Names: XPCC
• UniProt Entry Name: XPC_HUMAN

NCBI Description

The protein encoded by this gene is a key component of the XPC complex, which plays an important role in the early steps of global genome nucleotide excision repair (NER). The encoded protein is important for damage sensing and DNA binding, and shows a preference for single-stranded DNA. Mutations in this gene or some other NER components can result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2017]