Immunofluorescence (IF)
(Immunofluorescence staining of XPC in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human XPC polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Rabbit anti-Human XPC Polyclonal Antibody | anti-XPC antibody
Anti-XPC Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
XPC; Polyclonal Antibody; Anti-XPC Antibody; Rabbit Polyclonal; XPC Antibody; Rabbit PAb; Antigen Affinity Purified; xeroderma pigmentosum; complementation group C; Anti-p125 Antibody; Anti-RAD4 Antibody; Anti-XP3 Antibody; Anti-XPCC Antibody; anti-XPC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human XPC
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-XPC antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human XPC fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human XPC fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of XPC in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human XPC polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of XPC in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human XPC polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Related Product Information for anti-XPC antibody
XPC (XPC Complex Subunit, DNA Damage Recognition And Repair Factor) is a Protein Coding gene. The XPC gene, located on 3p25.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. The XPC protein is a component of a heterotrimeric complex that is essential for damage recognition in a nucleotide excision repair subpathway that operates throughout the genome. The encoded protein is important for damage sensing and DNA binding and shows a preference for single-stranded DNA. XPC is widely expressed in the ovary, kidney, and other tissues. Diseases associated with XPC include Xeroderma Pigmentosum, Complementation Group C, and Xeroderma Pigmentosum, Variant Type. Defects in XPC lead to carcinogenesis and the progression of many human cancers.
References
Zebian A, et al. (2019) Xpc beyond nucleotide excision repair and skin cancers. Mutat Res 782 108286.Wang H, et al. (2019) Xpc deficiency leads to centrosome amplification by inhibiting brca1 expression upon cisplatin-mediated DNA damage in human bladder cancer. Cancer Lett 444 136-146.Sugasawa K (2008) Xpc: Its product and biological roles. Adv Exp Med Biol 637 47-56.Bidon B, et al. (2018) Xpc is an rna polymerase ii cofactor recruiting atac to promoters by interacting with e2f1. Nat Commun 9 (1): 2610.
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Product Notes
The XPC (Catalog #AAA8109692) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-XPC Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's XPC can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the XPC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XPC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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