Goat XBP1 Polyclonal Antibody | anti-XBP1 antibody

Goat Polyclonal to Human XBP1

Cat. Num. AAA242009

• Gene Names: XBP1; XBP2; TREB5; XBP-1
• Reactivity: Gibbon, Chimpanzee, Gorilla, Human, Monkey, Orangutan; Predicted Reactivity: Mouse, Rat, Hamster (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: XBP1; Polyclonal Antibody; Goat Polyclonal to Human XBP1; Anti-XBP1 Antibody (Internal) IHC-plus; X-box binding protein 1; X-box-binding protein 1; XBP2; TREB5; XBP-1; Human XBP1; anti-XBP1 antibody

• Host: Goat
• Reactivity: Gibbon, Chimpanzee, Gorilla, Human, Monkey, Orangutan; Predicted Reactivity: Mouse, Rat, Hamster (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human XBP1. This antibody is expected to recognise isoform XBP1(S) (NP_001073007.1) only.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 261

• Applicable Applications for anti-XBP1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:4000), IHC-P (5 ug/ml), WB (1 - 3 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-NHSWEDTFANELFPQ from an internal region of human XBP1 (NP_001073007.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%); Mouse, Rat, Hamster, Panda (93%).
• Immunogen Type: Synthetic peptide
• Immunogen: XBP1 antibody was raised against synthetic peptide C-NHSWEDTFANELFPQ from an internal region of human XBP1 (NP_001073007.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%); Mouse, Rat, Hamster, Panda (93%); Pig, Opossum (87%); Bat, Turkey, Pufferfish (80%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-XBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-XBP1 antibody

XBP1 is a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. It is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator.

NCBI and Uniprot Product Information

• NCBI GI #: 14110395
• NCBI GeneID: 7494
• NCBI Accession #: NP_005071.2
• NCBI GenBank Nucleotide #: NM_005080.3
• UniProt Accession #: P17861; Q8WYK6; Q969P1; Q96BD7
• Molecular Weight: 40,148 Da
• NCBI Official Full Name: X-box-binding protein 1 isoform XBP1(U)
• NCBI Official Synonym Full Names: X-box binding protein 1
• NCBI Official Symbol: XBP1
• NCBI Official Synonym Symbols: XBP2; TREB5; XBP-1
• NCBI Protein Information: X-box-binding protein 1; tax-responsive element-binding protein 5
• UniProt Protein Name: X-box-binding protein 1
• Protein Family: X-box-binding protein
• UniProt Gene Name: XBP1
• UniProt Synonym Gene Names: TREB5; XBP2; XBP-1
• UniProt Entry Name: XBP1_HUMAN

NCBI Description

This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq, Jul 2008]

Uniprot Description

XBP1: a transcription factor essential for hepatocyte growth, the differentiation of plasma cells, immunoglobulin secretion, and the unfolded protein response (UPR). XBP1 mRNA is spliced by IRE1 during the UPR to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. Only the spliced form of XBP1 can activate the UPR efficiently. Activates UPR target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. Up-regulated by ATF6 via direct binding to the ERSE in response to endoplasmic reticulum stress. Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7). Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature. Two human isoforms are produced by alternative splicing. Isoform 1 is also known as XBP-1U. Isoform 2, also known as XBP-1S, is produced by IRE1 in response to endoplasmic reticulum stress. IRE1 cleaves a 26-bp fragment causing a frameshift of the mRNA transcript.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 22q12.1|22q12

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; endoplasmic reticulum; cytoplasm; integral to membrane; integral to endoplasmic reticulum membrane; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; protease binding; protein heterodimerization activity; chromatin DNA binding; ubiquitin protein ligase binding; estrogen receptor binding; transcription factor activity; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; phosphoinositide 3-kinase cascade; muscle development; apoptosis; exocrine pancreas development; positive regulation of transcription of target genes involved in unfolded protein response; regulation of protein stability; negative regulation of transcription from RNA polymerase II promoter; cellular response to glucose starvation; protein transport; serotonin secretion, neurotransmission; positive regulation of MHC class II biosynthetic process; positive regulation of transcription factor import into nucleus; angiogenesis; cell growth; response to electrical stimulus; positive regulation of autophagy; fatty acid biosynthetic process; positive regulation of TOR signaling pathway; response to drug; positive regulation of histone methylation; protein destabilization; unfolded protein response; organelle organization and biogenesis; cellular response to nutrient; positive regulation of immunoglobulin secretion; liver development; positive regulation of immunoglobulin production; cholesterol homeostasis; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; cellular response to insulin stimulus; positive regulation of T cell differentiation; fatty acid homeostasis; endothelial cell proliferation; positive regulation of fat cell differentiation; autophagy; positive regulation of B cell differentiation; neuron development; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; sterol homeostasis; negative regulation of apoptosis

Disease: Major Affective Disorder 7