Rabbit anti-Human, Mouse XAF-1 Polyclonal Antibody | anti-XAF-1 antibody

XAF-1 Antibody

Cat. Num. AAA9407882

• Gene Names: XAF1; BIRC4BP; XIAPAF1; HSXIAPAF1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: XAF-1; Polyclonal Antibody; XAF-1 Antibody; anti-XAF-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 317

• Applicable Applications for anti-XAF-1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1.
• Target Name: XAF-1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of XAF-1 in human spleen lysate with XAF-1 antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) ug/mL, respectively.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of human spleen tissue using XAF-1 antibody at 2 ug/mL.)

Related Product Information for anti-XAF-1 antibody

XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.

Product Categories/Family for anti-XAF-1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 1869901
• NCBI GeneID: 54739
• UniProt Accession #: Q6GPH4; Q6MZE8; Q8N557; Q99982; A2T931; A2T932; A8K2L1; A8K9Y3; D3DTM6
• Molecular Weight: 34,543 Da
• NCBI Official Full Name: XIAP associated factor-1 (ZAP-1)
• NCBI Official Synonym Full Names: XIAP associated factor 1
• NCBI Official Symbol: XAF1
• NCBI Official Synonym Symbols: BIRC4BP; XIAPAF1; HSXIAPAF1
• NCBI Protein Information: XIAP-associated factor 1
• UniProt Protein Name: XIAP-associated factor 1
• UniProt Gene Name: XAF1
• UniProt Synonym Gene Names: BIRC4BP; XIAPAF1
• UniProt Entry Name: XAF1_HUMAN

NCBI Description

This gene encodes a protein which binds to and counteracts the inhibitory effect of a member of the IAP (inhibitor of apoptosis) protein family. IAP proteins bind to and inhibit caspases which are activated during apoptosis. The proportion of IAPs and proteins which interfere with their activity, such as the encoded protein, affect the progress of the apoptosis signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

BIRC4BP: Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti- caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha- induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Apoptosis

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: mitochondrion; cytosol; nucleus

Molecular Function: zinc ion binding

Biological Process: apoptosis; cytokine and chemokine mediated signaling pathway; negative regulation of protein complex assembly