Rabbit anti-Human XIAP Associated Factor 1 Polyclonal Antibody | anti-XAF1 antibody

XIAP Associated Factor 1 (XAF1, X Chromosome linked Inhibitor of Apoptosis Associated Factor 1)

Cat. Num. AAA612734

• Gene Names: XAF1; BIRC4BP; XIAPAF1; HSXIAPAF1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: XIAP Associated Factor 1; Polyclonal Antibody; XIAP Associated Factor 1 (XAF1; X Chromosome linked Inhibitor of Apoptosis Associated Factor 1); Anti -XIAP Associated Factor 1 (XAF1; anti-XAF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human XIAP Associated Factor 1 (XAF1).
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-XAF1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-2ug/ml; A band of 34kD should be detected.; Immunohistochemistry: 1:100
• Immunogen: KLH-conjugated synthetic peptides corresponding to aa201-218 and aa301-317 of human XAF1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of XAF1 in human liver lysates usingMBS612734 at 1 ug/ml.)

Related Product Information for anti-XAF1 antibody

The inhibitors of apoptosis (IAP) proteins prevent cell death both in vitro and in vivo by virtue of inhibition of distinct caspases. The X-linked Inhibitor of Apoptosis, XIAP, is a key member of the IAP family of proteins. The caspase inhibiting activity of XIAP is negatively regulated by at least two XIAP-interacting proteins, XIAP-associated factor 1 (XAF1) and Smac/DIABLO. XAF1 binds to XIAP and antagonizes caspase inhibitory activities of XIAP. Expression of XAF1 triggers a redistribution of XIAP from the cytosol to the nucleus. XAF1 is ubiquitously expressed in normal tissues, but is present at low or undetectable levels in many different cancer cell lines.

Product Categories/Family for anti-XAF1 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 40288191
• NCBI GeneID: 54739
• NCBI Accession #: NP_059993.2
• NCBI GenBank Nucleotide #: NM_017523.2
• UniProt Accession #: Q6GPH4; Q6MZE8; Q8N557; Q99982; A2T931; A2T932; A8K2L1; A8K9Y3; D3DTM6
• Molecular Weight: 34,626 Da
• NCBI Official Full Name: XIAP-associated factor 1 isoform 1
• NCBI Official Synonym Full Names: XIAP associated factor 1
• NCBI Official Symbol: XAF1
• NCBI Official Synonym Symbols: BIRC4BP; XIAPAF1; HSXIAPAF1
• NCBI Protein Information: XIAP-associated factor 1; BIRC4 binding protein; BIRC4-binding protein
• UniProt Protein Name: XIAP-associated factor 1
• Protein Family: XIAP-associated factor
• UniProt Gene Name: XAF1
• UniProt Synonym Gene Names: BIRC4BP; XIAPAF1
• UniProt Entry Name: XAF1_HUMAN

NCBI Description

X-linked inhibitor of apoptosis (XIAP; MIM 300079) is a potent member of the IAP family. All members of this family possess baculoviral IAP (BIR) repeats, cysteine-rich domains of approximately 80 amino acids that bind and inhibit caspases (e.g., CASP3; MIM 600636). XIAP has 3 BIR domains and a C-terminal RING zinc finger that possesses E3 ubiquitin ligase (see MIM 601623) activity. XAF1 antagonizes the anticaspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells (Liston et al., 2001 [PubMed 11175744]).[supplied by OMIM]

Uniprot Description

BIRC4BP: Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti- caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha- induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Tumor suppressor

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: mitochondrion; nucleus; cytosol

Molecular Function: zinc ion binding

Biological Process: apoptosis; cytokine and chemokine mediated signaling pathway; negative regulation of protein complex assembly