Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Rabbit XAB2 Polyclonal Antibody | anti-XAB2 antibody
XAB2 Antibody
Gene Names
XAB2; HCNP; HCRN; SYF1; NTC90
Reactivity
Human, Mouse, MonkeyPredicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
XAB2; Polyclonal Antibody; XAB2 Antibody; 0610041O14Rik; adapter protein ATH 55; Adapter protein ATH-55; Ath55; AV025587; Crn related protein kim1; crn-related protein kim1; DKFZp762C1015; HCNP; HCNP protein; HCRN; KIAA1177; NTC90; PP3898; Pre-mRNA-splicing factor SYF1; Protein HCNP; RNA splicing factor; SYF1; SYF1 homolog; SYF1 pre mRNA splicing factor; SYF1_HUMAN; Xab2; XPA binding protein 2; XPA-binding protein 2; anti-XAB2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Monkey
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
XAB2 Antibody detects endogenous levels of XAB2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
855
Applicable Applications for anti-XAB2 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human XAB2.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using XAB2 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using XAB2 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using XAB2 Antibody. Lane 1: 3T3, treated with blocking peptide; Lane 2: 3T3; Lane 3: Mouse spleen; Lane 4: COS7; Lane 5: Colo205.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using XAB2 Antibody. Lane 1: 3T3, treated with blocking peptide; Lane 2: 3T3; Lane 3: Mouse spleen; Lane 4: COS7; Lane 5: Colo205.)
Related Product Information for anti-XAB2 antibody
Description: Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:28502770, PubMed:28076346). Involved in transcription-coupled repair (TCR), transcription and pre-mRNA splicing (PubMed:10944529, PubMed:17981804).
Subunit Structure: Associates with RNA polymerase II, the TCR-specific proteins CKN1/CSA and ERCC6/CSB, and XPA (PubMed:10944529). Identified in the spliceosome C complex (PubMed:11991638, PubMed:28502770, PubMed:28076346). Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE (PubMed:17981804). Identified in a pentameric intron-binding (IB) complex composed of AQR, XAB2, ISY1, ZNF830 and PPIE that is incorporated into the spliceosome as a preassembled complex (PubMed:25599396). The IB complex does not contain PRPF19 (PubMed:25599396).
Similarity: Belongs to the crooked-neck family.
Subunit Structure: Associates with RNA polymerase II, the TCR-specific proteins CKN1/CSA and ERCC6/CSB, and XPA (PubMed:10944529). Identified in the spliceosome C complex (PubMed:11991638, PubMed:28502770, PubMed:28076346). Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE (PubMed:17981804). Identified in a pentameric intron-binding (IB) complex composed of AQR, XAB2, ISY1, ZNF830 and PPIE that is incorporated into the spliceosome as a preassembled complex (PubMed:25599396). The IB complex does not contain PRPF19 (PubMed:25599396).
Similarity: Belongs to the crooked-neck family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 100 kDa
Predicted: 101 kDa
Predicted: 101 kDa
NCBI Official Full Name
Pre-mRNA-splicing factor SYF1
NCBI Official Synonym Full Names
XPA binding protein 2
NCBI Official Symbol
XAB2
NCBI Official Synonym Symbols
HCNP; HCRN; SYF1; NTC90
NCBI Protein Information
pre-mRNA-splicing factor SYF1
UniProt Protein Name
Pre-mRNA-splicing factor SYF1
UniProt Gene Name
XAB2
UniProt Synonym Gene Names
HCNP; KIAA1177; SYF1
UniProt Entry Name
SYF1_HUMAN
Uniprot Description
XAB2: Involved in transcription-coupled repair (TCR), transcription and pre-mRNA splicing. Belongs to the crooked-neck family.
Protein type: Spliceosome; RNA splicing
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: nucleoplasm; membrane; nucleus
Molecular Function: protein binding
Biological Process: nuclear mRNA splicing, via spliceosome; blastocyst development; transcription, DNA-dependent; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; DNA repair
Research Articles on XAB2
Similar Products
Product Notes
The XAB2 xab2 (Catalog #AAA9611549) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The XAB2 Antibody reacts with Human, Mouse, Monkey Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's XAB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the XAB2 xab2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XAB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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