Rabbit WSTF Polyclonal Antibody | anti-baz1b antibody

WSTF (Williams Syndrome Transcription Factor, BAZB1, Bromodomain Adjacent to Zinc Finger Domain 1B, WBSCR9, Williams-Beuren Syndrome Chromosome Region 9, WBSC10, Williams-Beuren Syndrome Chromosone)

Cat. Num. AAA610881

• Gene Names: baz1b; wstf; wbscr9; wbscr10
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: WSTF; Polyclonal Antibody; WSTF (Williams Syndrome Transcription Factor; BAZB1; Bromodomain Adjacent to Zinc Finger Domain 1B; WBSCR9; Williams-Beuren Syndrome Chromosome Region 9; WBSC10; Williams-Beuren Syndrome Chromosone); Anti -WSTF (Williams Syndrome Transcription Factor; anti-baz1b antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total human WSTF protein. Does not crossreact with other related transcriptional factors. Species Crossreactivity: Human, monkey, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-baz1b antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide derived from the sequence of human WSTF (KLH coupled) (Gene: WBSCR9).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of lysates from various cell lines, using MBS610881.)

Testing Data

Related Product Information for anti-baz1b antibody

The human WSTF gene is located within the common Williams Syndrome (WS) deletion area at chromosome 7q11.23. Several WSTF gene products have been detected with little difference in length of polypeptides (1-3). Functional motifs identified by sequence-homology searches include a PHD-type zinc finger motif followed by a bromodomain. Both motifs are found in many transcriptional factors, suggesting that WSTF may function as a transcription factor. A Drosophila gene (acf1) was cloned, which encodes two forms of Acf1 proteins with molecular weight 170kD and 185kD, respectively (4). It was demonstrated that Acf1 is structurally related with the human WSTF gene. Acf1 forms a complex with another protein, ISWI, and functions in the ATP-dependent catalysis of chromatin assembly (4).

Product Categories/Family for anti-baz1b antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 116487535
• NCBI GeneID: 443594
• UniProt Accession #: A8DZJ1; Q6GQ06; A0JMY1
• Molecular Weight: 166,153 Da
• NCBI Official Full Name: Wstf protein
• NCBI Official Synonym Full Names: bromodomain adjacent to zinc finger domain, 1B
• NCBI Official Symbol: baz1b
• NCBI Official Synonym Symbols: wstf; wbscr9; wbscr10
• NCBI Protein Information: tyrosine-protein kinase BAZ1B; Williams syndrome transcription factor homolog; Bromodomain adjacent to zinc finger domain protein 1B
• UniProt Protein Name: Tyrosine-protein kinase BAZ1B
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: baz1b
• UniProt Synonym Gene Names: wstf
• UniProt Entry Name: BAZ1B_XENLA

Uniprot Description

Function: Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR). In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Cofactor: Manganese

Subunit structure: Interacts with smarca5/ snf2h; the interaction is direct and forms the WICH complex. Component of the B-WICH complex. Component of the WINAC complex

Subcellular location: Nucleus

By similarity. Note: Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase

Tissue specificity: Highly expressed in the neural tube. Ref.1

Developmental stage: Detected maternally in mature unfertilized oocytes and ubiquitously expressed until embryonic stage 16. In stage 17/18, expression becomes restricted to the closing neural tube. Ref.1

Domain: The bromo domain mediates the specific interaction with acetylated histones

Sequence similarities: Belongs to the WAL family. BAZ1B subfamily.Contains 1 bromo domain.Contains 1 DDT domain.Contains 1 PHD-type zinc finger.Contains 1 WAC domain.

Sequence caution: The sequence AAH72944.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.