Mouse NOV ELISA Kit | NOV elisa kit

Mouse NOV ELISA Kit

Cat. Num. AAA8248453

• Gene Names: Nov; CCN3; C130088N23Rik
• Reactivity: Mouse
• Synonyms: NOV; Mouse NOV ELISA Kit; CCN3; IGFBP9; NOVH; Protein NOV homolog; NovH; CCN family member 3; Insulin-like growth factor-binding protein 9; IBP-9; IGF-binding protein 9; IGFBP-9; Nephroblastoma-overexpressed gene protein homolog; NOV elisa kit

• Reactivity: Mouse
• Specificity: The Mouse NOV ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse NOV proteins within the range of 31.2 pg/ml - 2000 pg/ml.
• Sequence Length: 354

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOV elisa kit

Principle of the Assay: The Mouse NOV ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse NOV in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse NOV coated on a 96-well plate. Standards and samples are pipetted into the wells and NOV present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse NOV antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NOV bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: NOV (nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the identification of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.

NCBI and Uniprot Product Information

• NCBI GI #: 6754876
• NCBI GeneID: 18133
• NCBI Accession #: NP_035060.1
• NCBI GenBank Nucleotide #: NM_010930.4
• UniProt Accession #: Q64299; Q8CA67
• Molecular Weight: 38,928 Da
• NCBI Official Full Name: protein NOV homolog
• NCBI Official Synonym Full Names: nephroblastoma overexpressed gene
• NCBI Official Symbol: Nov
• NCBI Official Synonym Symbols: CCN3; C130088N23Rik
• NCBI Protein Information: protein NOV homolog
• UniProt Protein Name: Protein NOV homolog
• Protein Family: Novel plant
• UniProt Gene Name: Nov
• UniProt Synonym Gene Names: Ccn3; NovH
• UniProt Entry Name: NOV_MOUSE

Uniprot Description

NOV: Immediate-early protein likely to play a role in cell growth regulation. Belongs to the CCN family. Expression is down-regulated by WT1. Interacts with FBLN1.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: axon; cell soma; cytoplasm; dendrite; extracellular matrix; extracellular region; gap junction; intracellular membrane-bound organelle; proteinaceous extracellular matrix

Molecular Function: heparin binding; integrin binding; Notch binding

Biological Process: angiogenesis; cell adhesion; cell adhesion mediated by integrin; chondrocyte differentiation; negative regulation of cell growth; negative regulation of inflammatory response; negative regulation of insulin secretion; negative regulation of NF-kappaB import into nucleus; positive regulation of Notch signaling pathway; regulation of gene expression; smooth muscle cell migration; smooth muscle cell proliferation