Immunofluorescence (IF)
(Immunofluorescent staining of chick embryos usingMBS617938.)
Rabbit anti-Chicken Wnt6 Polyclonal Antibody | anti-Wnt6 antibody
Wnt6 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family, Member 6 (Wnt6) (AP)
Reactivity
Chicken
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Epitope-affinity purified from rabbit antiserum.
Synonyms
Wnt6; Polyclonal Antibody; Wnt6 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family; Member 6 (Wnt6) (AP); anti-Wnt6 antibody
Host
Rabbit
Reactivity
Chicken
Clonality
Polyclonal
Isotype
IgG
Specificity
Reacts with the chicken Wnt6 protein. On Western blots, it identifies a band at ~35kD and one at ~45kD. The two bands may represent different isoforms or post-translational modification states. Immunohistochemical and immunofluoresent staining using this antibody is observed in non-neural ectoderm of chick embryos from stages 8 to 14, consistent with the reported expression of Wnt6 mRNA as determined by in situ hybridization. Additional staining is found in ectodermal tissues caudal to the otic level in heart-and gut-forming regions. Reactivity has been confirmed with stage 8-20 chicken embryo homogenates.
Purity/Purification
Epitope-affinity purified from rabbit antiserum.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-Wnt6 antibody
Immunohistochemistry (IHC) Frozen, Immunofluorescence (IF), Western Blot (WB)
Application Notes
IHC-F: 2-3ug/ml
IF: 2-3ug/ml
WB: 1-3ug/ml
Applications are based on unconjugated antibody.
IF: 2-3ug/ml
WB: 1-3ug/ml
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide derived from an internal region of the chicken Wnt6 protein.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunofluorescence (IF)
(Immunofluorescent staining of chick embryos usingMBS617938.)
Immunofluorescence (IF)
(Immunofluorescent staining of chick embryos usingMBS617938.)
Product Categories/Family for anti-Wnt6 antibody
References
1. Cadigan KM and Nusse R. Genes Dev 11:3286-3305, 1997. 2. Miller JR, et al. Oncogene 18:7860-7872, 1999. 3. Polakis P. Genes Dev 14:1837-1851, 2000. 4. Adler, PN and Lee H. Curr Opin Cell Biol 13:635-640, 2001. 5. Kuhl M, et al. Trends Genet 16:279-283, 2000. 6. Rodriguez-Niedenfuhr M, et al. Anat Embryol (Berl) 206:447-451, 2003. 7. Garcia-Castro MI, et al. Science 297:848-851, 2002. 8. Schubert FR, et al. Mech Dev 114:143-148, 2002. 9. Dietrich S, et al. Development 125:2235-2249, 1998.
Similar Products
Product Notes
The Wnt6 (Catalog #AAA6506224) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Wnt6 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family, Member 6 (Wnt6) (AP) reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Wnt6 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Frozen, Immunofluorescence (IF), Western Blot (WB). IHC-F: 2-3ug/ml IF: 2-3ug/ml WB: 1-3ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Wnt6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wnt6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
