Western Blot (WB)
(Western Blot of WNT-1-pCMV-Tag2A-transfected HEK293 cell lysate is used as the positive control for over-expressed protein.)
Rabbit anti-Human, Mouse Wnt1 Polyclonal Antibody | anti-Wnt1 antibody
Wnt1 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member) (MaxLight 650)
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Purified by Epitope Affinity Chromatography.
Synonyms
Wnt1; Polyclonal Antibody; Wnt1 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member) (MaxLight 650); anti-Wnt1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Reacts with the human and mouse Wnt1 proteins. On Western blots, it identifies a single band at ~41kD. A band of unknown origin at ~80-90kD is sometimes observed; this may represent a dimerized form of Wnt1. Reactivity has been confirmed with Wnt1-transfected HEK293 cells, GIST882 and SW480 cell lysates, and mouse MS-1 cell lysates.
Purity/Purification
Purified by Epitope Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight650.
Applicable Applications for anti-Wnt1 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IP: 7ug/reaction
WB: 0.5-2ug/ml
Applications are based on unconjugated antibody.
WB: 0.5-2ug/ml
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide derived from the C-terminal region of the human Wnt1 protein.
Conjugate
MaxLight650
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot of WNT-1-pCMV-Tag2A-transfected HEK293 cell lysate is used as the positive control for over-expressed protein.)
Western Blot (WB)
(Western Blot of WNT-1-pCMV-Tag2A-transfected HEK293 cell lysate is used as the positive control for over-expressed protein.)
Western Blot (WB)
(Western Blot of endogenous Wnt1 using W3006 on GIST882 gastro-intestinal solid tumor, MS-1 mouse pancreatic endothelium and SW480 colorectal adenocarcinoma cell lysates)
Western Blot (WB)
(Western Blot of endogenous Wnt1 using W3006 on GIST882 gastro-intestinal solid tumor, MS-1 mouse pancreatic endothelium and SW480 colorectal adenocarcinoma cell lysates)
Product Categories/Family for anti-Wnt1 antibody
Similar Products
Product Notes
The Wnt1 (Catalog #AAA6506155) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Wnt1 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member) (MaxLight 650) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Wnt1 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). IP: 7ug/reaction WB: 0.5-2ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Wnt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wnt1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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