Western Blot (WB)
(Western blot analysis of WNT5B expression in HepG2 (A), SHSY5Y (B), mouse liver (C) whole cell lysates.)
Rabbit anti-Human, Mouse WNT5B Polyclonal Antibody | anti-WNT5B antibody
Anti-WNT5B Antibody
Western Blot (WB)
(Western blot analysis of WNT5B expression in HepG2 (A), SHSY5Y (B), mouse liver (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of WNT5B expression in HepG2 (A), SHSY5Y (B), mouse liver (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of WNT5B staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of WNT5B staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
Uniprot Description
WNT5B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Interacts with PORCN. Belongs to the Wnt family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 12p13.3
Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane
Molecular Function: frizzled binding; receptor binding
Biological Process: cell fate commitment; chondrocyte differentiation; neuron differentiation; positive regulation of cell migration; Wnt receptor signaling pathway
Research Articles on WNT5B
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Product Notes
The WNT5B wnt5b (Catalog #AAA8247740) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-WNT5B Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's WNT5B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1/500 - 1/2000, Immunohistochemistry: 1/50 - 1/100. Researchers should empirically determine the suitability of the WNT5B wnt5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNT5B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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