Rabbit anti-Human, mouse Map3k5 Polyclonal Antibody | anti-Map3k5 antibody

Mouse Map3k5 Antibody (C-term)

Cat. Num. AAA9213180

• Gene Names: Map3k5; ASK; ASK1; Mekk5; MAPKKK5; 7420452D20Rik
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Map3k5; Polyclonal Antibody; Mouse Map3k5 Antibody (C-term); Mitogen-activated protein kinase kinase kinase 5; Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; Ask1; Mekk5; anti-Map3k5 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Mouse Map3k5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1175-1203 amino acids from the C-terminal region of mouse Map3k5.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.29 (varies by lot)
• Sequence Positions: 1175-1203
• Sequence Length: 1,380

• Applicable Applications for anti-Map3k5 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: MOUSE
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of Map3k5 (arrow) using rabbit polyclonal Mouse Map3k5 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map3k5 gene.)

Western Blot (WB)

(Mouse Map3k5 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Map3k5 antibody detected the Map3k5 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Map3k5 Antibody(C-term) (MBS9213180)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map3k5 Antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Map3k5 antibody

Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death (By similarity).

Product Categories/Family for anti-Map3k5 antibody

Cardiovascular; Cell Biology; Amyotrophic Lateral Sclerosis (ALS); Phospho Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 171846249
• NCBI GeneID: 26408
• NCBI Accession #: NP_032606.4
• NCBI GenBank Nucleotide #: NM_008580.4
• UniProt Accession #: O35099; Q14AY5
• Molecular Weight: 154512
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 5
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 5
• NCBI Official Symbol: Map3k5
• NCBI Official Synonym Symbols: ASK; ASK1; Mekk5; MAPKKK5; 7420452D20Rik
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 5
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 5
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: Map3k5
• UniProt Synonym Gene Names: Ask1; Mekk5; ASK-1; MEK kinase 5; MEKK 5
• UniProt Entry Name: M3K5_MOUSE

Uniprot Description

ASK1: a MAP kinase kinase kinase of the STE11 family, apoptosis signal-regulating kinase 1 (ASK1) plays essential roles in stress-induced apoptosis. Activated by various stressors, including oxidative stress, endoplasmic reticulum stress, and calcium overload, as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) and lipopolysaccharide (LPS). In turn, ASK1 phosphorylates and activates two different subgroups of MAP kinase kinases, MKK4/SEK1 and MKK3/MKK6, which in turn activate stress-activated protein kinases (JNKs) and p38 subgroups of MAP kinases, respectively. Overexpression activates JNK and p38 and induces apoptosis in several cell types through signals involving the mitochondrial cell death pathway. Embryonic fibroblasts or primary neurons derived from ASK1-/- mice are resistant to stress-induced JNK and p38 activation as well as cell death. Oxidative stress induces the activation of ASK1, which correlate with ASK1 activity and ASK1-dependent apoptosis. Required for the innate immune response, which is essential for host defense against a wide range of pathogens.

Protein type: Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.25; STE group; STE11 family

Cellular Component: endoplasmic reticulum; cytoplasm; intracellular

Molecular Function: protein homodimerization activity; metal ion binding; magnesium ion binding; nucleotide binding; caspase activator activity; protein kinase binding; protein phosphatase binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding

Biological Process: caspase activation; positive regulation of JNK activity; activation of MAPKK activity; apoptosis; immune system process; activation of MAPK activity; positive regulation of apoptosis; MAPKKK cascade; positive regulation of caspase activity; protein amino acid phosphorylation; JNK cascade; innate immune response; positive regulation of protein amino acid phosphorylation; phosphorylation; induction of apoptosis by oxidative stress