Western Blot (WB)
(Western Blot of Hela cell line lysates (35ug/lane) usingMBS6002362. This demonstrates that MBS6002362 detected the WNT5A protein (arrow).)
Rabbit WNT5A Polyclonal Antibody | anti-WNT5A antibody
WNT5A, ID (WNT5A, Protein Wnt-5a) (MaxLight 650)
Western Blot (WB)
(Western Blot of Hela cell line lysates (35ug/lane) usingMBS6002362. This demonstrates that MBS6002362 detected the WNT5A protein (arrow).)
Western Blot (WB)
(Western Blot of Hela cell line lysates (35ug/lane) usingMBS6002362. This demonstrates that MBS6002362 detected the WNT5A protein (arrow).)
Testing Data
(All lanes: MBS6002362 (1:500) Lane 1: Hela whole cell lysate Lane 2: mouse brain lysate Lane 3: mouse heart lysate Lane 4: PANC-1 whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 42kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data
(All lanes: MBS6002362 (1:500) Lane 1: Hela whole cell lysate Lane 2: mouse brain lysate Lane 3: mouse heart lysate Lane 4: PANC-1 whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 42kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Immunohistochemistry of formalin fixed and paraffin embedded human lung carcinoma using MBS6002362 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MBS6002362 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry of formalin fixed and paraffin embedded human lung carcinoma using MBS6002362 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MBS6002362 for immunohistochemistry.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Hela cells using MBS6002362 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Hela cells using MBS6002362 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
Research Articles on WNT5A
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Product Notes
The WNT5A wnt5a (Catalog #AAA6363728) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WNT5A, ID (WNT5A, Protein Wnt-5a) (MaxLight 650) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's WNT5A can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the WNT5A wnt5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNT5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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