Typical Testing Data/Standard Curve (for reference only)
Human VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2) CLIA Kit | VEGFR-2/KDR clia kit
Human VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to VEGFR-2/KDR. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for VEGFR-2/KDR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VEGFR-2/KDR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of VEGFR-2/KDR. You can calculate the concentration of VEGFR-2/KDR in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]
Uniprot Description
VEGFR2: a receptor tyrosine kinase of the VEGFR family. High affinity receptor for VEGF and VEGF-C. Ligand binding induces autophosphorylation and activation. Activated receptor recruits proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. Plays a key role in vascular development and regulation of vascular permeability.
Protein type: Protein kinase, TK; Protein kinase, tyrosine (receptor); Membrane protein, integral; EC 2.7.10.1; Kinase, protein; TK group; VEGFR family
Chromosomal Location of Human Ortholog: 4q11-q12
Cellular Component: cell junction; cytoplasmic membrane-bound vesicle; early endosome; endoplasmic reticulum; endosome; external side of plasma membrane; extracellular region; Golgi apparatus; integral to plasma membrane; lipid raft; nucleus; plasma membrane
Molecular Function: ATP binding; growth factor binding; Hsp90 protein binding; integrin binding; protein binding; protein-tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor receptor activity
Biological Process: alveolus development; angiogenesis; branching morphogenesis of a tube; calcium ion homeostasis; cell fate commitment; cell maturation; cell migration during sprouting angiogenesis; embryonic hemopoiesis; endothelial cell differentiation; extracellular matrix organization and biogenesis; lymph vessel development; negative regulation of apoptosis; ovarian follicle development; peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of focal adhesion formation; positive regulation of macroautophagy; positive regulation of MAPKKK cascade; positive regulation of mesenchymal cell proliferation; positive regulation of mitochondrial depolarization; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of positive chemotaxis; positive regulation of protein amino acid phosphorylation; post-embryonic camera-type eye morphogenesis; protein amino acid autophosphorylation; regulation of cell shape; regulation of endothelial cell differentiation; surfactant homeostasis; transmembrane receptor protein tyrosine kinase signaling pathway; vascular endothelial growth factor receptor signaling pathway; vasculogenesis; viral reproduction
Disease: Hemangioma, Capillary Infantile
Research Articles on VEGFR-2/KDR
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Product Notes
The Human VEGFR-2/KDR kdr (Catalog #AAA2532594) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2532594 CLIA Kit recognizes Human VEGFR-2/KDR. It is sometimes possible for the material contained within the vial of "VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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