Rabbit WNT5A Polyclonal Antibody | anti-WNT5A antibody

WNT5A, ID (WNT5A, Protein Wnt-5a) (FITC)

Cat. Num. AAA6363723

• Gene Names: WNT5A; hWNT5A
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: WNT5A; Polyclonal Antibody; ID (WNT5A; Protein Wnt-5a) (FITC); Protein Wnt-5a; anti-WNT5A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human WNT5A. Species Crossreactivity: mouse, rat
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-WNT5A antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 185-213 in the central region of human WNT5A, Swiss/UniProt Accession: P41221
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot of Hela cell line lysates (35ug/lane) usingMBS6002362. This demonstrates that MBS6002362 detected the WNT5A protein (arrow).)

Testing Data

(All lanes: MBS6002362 (1:500) Lane 1: Hela whole cell lysate Lane 2: mouse brain lysate Lane 3: mouse heart lysate Lane 4: PANC-1 whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 42kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry of formalin fixed and paraffin embedded human lung carcinoma using MBS6002362 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MBS6002362 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Hela cells using MBS6002362 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-WNT5A antibody

Antibodies; Wnt Proteins

References

He, X., et al. Science 275(5306):1652-1654(1997). Danielson, K.G., et al. J. Biol. Chem. 270(52):31225-31234(1995) Smolich, B.D., et al. Mol. Biol. Cell 4(12):1267-1275(1993) Clark, C.C., et al. Genomics 18(2):249-260(1993).

NCBI and Uniprot Product Information

• NCBI GI #: 40806205
• NCBI GeneID: 7474
• NCBI Accession #: NP_003383.2
• NCBI GenBank Nucleotide #: NM_003392.4
• UniProt Accession #: P41221; Q6P278; A8K4A4
• Molecular Weight: 40,887 Da
• NCBI Official Full Name: protein Wnt-5a isoform 1
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 5A
• NCBI Official Symbol: WNT5A
• NCBI Official Synonym Symbols: hWNT5A
• NCBI Protein Information: protein Wnt-5a; WNT-5A protein
• UniProt Protein Name: Protein Wnt-5a
• Protein Family: Protein
• UniProt Gene Name: WNT5A
• UniProt Entry Name: WNT5A_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]